Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10193(B)] to LEPRE1/P3H1
  • Suitable for: ELISA, WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-LEPRE1/P3H1 antibody [EPR10193(B)]
    See all LEPRE1/P3H1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10193(B)] to LEPRE1/P3H1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human LEPRE1/P3H1. The exact sequence is proprietary.

  • Positive control

    • HeLa, HepG2, A549 and Human placenta lysates; HepG2 and A549 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as LEPRE1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • ELISA - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
    ELISA - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

    ELISA using ab154799 at varying antibody concentrations and antigen concentration at 1000 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • Western blot - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
    Western blot - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
    All lanes : Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799) at 1/1000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : HepG2 lysate
    Lane 3 : A549 lysate
    Lane 4 : Human placenta lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 83 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
    Immunocytochemistry/ Immunofluorescence - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling LEPRE1/P3H1 with Purified ab154799 at 1/500 dilution (5µg/ml). Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
    Immunocytochemistry/ Immunofluorescence - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

    Immunofluorescence analysis of HepG2 cells labeling LEPRE1/P3H1 with ab154799 at 1/250 dilution.

  • Flow Cytometry - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
    Flow Cytometry - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

    Flow cytometric analysis of permeabilized A549 cells labeling LEPRE1/P3H1 with ab154799 at 1/10 dilution (red) or a negative rabbit IgG (green).

  • Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
    Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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