Antibodies, Proteins, Kits and Reagents for Life Science

318 288 ₸ Product size

100 µl 318 288 ₸

Order now and get it on Tuesday March 09, 2021

Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10193(B)] to LEPRE1/P3H1
Suitable for: ELISA, WB, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-LEPRE1/P3H1 antibody [EPR10193(B)]
See all LEPRE1/P3H1 primary antibodies
Description

Rabbit monoclonal [EPR10193(B)] to LEPRE1/P3H1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human LEPRE1/P3H1. The exact sequence is proprietary.
Positive control
- HeLa, HepG2, A549 and Human placenta lysates; HepG2 and A549 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product was previously labelled as LEPRE1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10193(B)
Isotype

IgG
Research areas

ELISA - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

ELISA using ab154799 at varying antibody concentrations and antigen concentration at 1000 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
Western blot - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

All lanes : Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799) at 1/1000 dilution

Lane 1 : HeLa lysate
Lane 2 : HepG2 lysate
Lane 3 : A549 lysate
Lane 4 : Human placenta lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 83 kDa
Immunocytochemistry/ Immunofluorescence - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling LEPRE1/P3H1 with Purified ab154799 at 1/500 dilution (5µg/ml). Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor^®488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Immunocytochemistry/ Immunofluorescence - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

Immunofluorescence analysis of HepG2 cells labeling LEPRE1/P3H1 with ab154799 at 1/250 dilution.
Flow Cytometry - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

Flow cytometric analysis of permeabilized A549 cells labeling LEPRE1/P3H1 with ab154799 at 1/10 dilution (red) or a negative rabbit IgG (green).
Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com