Anti-LAPTM4B antibody [CL5289] (ab242376)
Key features and details
- Mouse monoclonal [CL5289] to LAPTM4B
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-LAPTM4B antibody [CL5289]
See all LAPTM4B primary antibodies -
Description
Mouse monoclonal [CL5289] to LAPTM4B -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human LAPTM4B aa 200-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q86VI4 -
Positive control
- IHC-P: Human heart muscle tissue, kidney tissue and cerebral cortex tissue. WB: HeLa whole cell lysate. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL5289 -
Isotype
IgG1 -
Research areas
Images
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Anti-LAPTM4B antibody [CL5289] (ab242376) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 35 kDa
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Paraffin-embedded human cerebral cortex tissue stained for LAPTM4B using ab242376 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence anaysis of A431 cells labeling LAPTM4B with ab242376 at 2-10 µg/mL. Cells were fixed with PFA and permeabilised with Triton X-100. The data is showing specific staining in vesicles and the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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Paraffin-embedded human kidney tissue stained for LAPTM4B using ab242376 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human heart muscle tissue stained for LAPTM4B using ab242376 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human pancreas tissue stained for LAPTM4B using ab242376 at 1/500 dilution in immunohistochemical analysis.