Antibodies, Proteins, Kits and Reagents for Life Science

Anti-LAPTM4B antibody [CL5289] (ab242376)

Key features and details

Mouse monoclonal [CL5289] to LAPTM4B
Suitable for: ICC/IF, IHC-P, WB
Reacts with: Human
Isotype: IgG1

Product name

Anti-LAPTM4B antibody [CL5289]
See all LAPTM4B primary antibodies
Description

Mouse monoclonal [CL5289] to LAPTM4B
Host species

Mouse
Tested applications

Suitable for: ICC/IF, IHC-P, WBmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human LAPTM4B aa 200-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q86VI4

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Positive control
- IHC-P: Human heart muscle tissue, kidney tissue and cerebral cortex tissue. WB: HeLa whole cell lysate. ICC/IF: A431 cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

CL5289
Isotype

IgG1
Research areas

Western blot - Anti-LAPTM4B antibody [CL5289] (ab242376)

Anti-LAPTM4B antibody [CL5289] (ab242376) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAPTM4B antibody [CL5289] (ab242376)

Paraffin-embedded human cerebral cortex tissue stained for LAPTM4B using ab242376 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-LAPTM4B antibody [CL5289] (ab242376)

Immunocytochemistry/ Immunofluorescence anaysis of A431 cells labeling LAPTM4B with ab242376 at 2-10 µg/mL. Cells were fixed with PFA and permeabilised with Triton X-100. The data is showing specific staining in vesicles and the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAPTM4B antibody [CL5289] (ab242376)

Paraffin-embedded human kidney tissue stained for LAPTM4B using ab242376 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAPTM4B antibody [CL5289] (ab242376)

Paraffin-embedded human heart muscle tissue stained for LAPTM4B using ab242376 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAPTM4B antibody [CL5289] (ab242376)

Paraffin-embedded human pancreas tissue stained for LAPTM4B using ab242376 at 1/500 dilution in immunohistochemical analysis.

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