Immunohistochemical analysis (Formalin/PFA-fixed paraffin-embedded sections) of rat lymph node tissue labeling LAP1. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 at 1/50 dilution overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

Immunocytochemical analysis of NS-1 (Mouse myeloma cell line) cells labeling LAP1 with ab2737 at 1/100 dilution.

Immunohistochemical analysis (Formalin/PFA-fixed paraffin-embedded sections) rat breast tissue labeling LAP1. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 1/50 dilution overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

Immunohistochemical analysis of (Formalin/PFA-fixed paraffin-embedded sections) rat colon tissue labeling LAP1. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 at 1/50 dilution overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.