Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LAP1 antibody [RL13] (ab2737)

Key features and details

  • Mouse monoclonal [RL13] to TOR1AIP1
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Mouse, Rat
  • Isotype: IgG1

Overview

  • Product name

    Anti-TOR1AIP1 antibody [RL13]
    See all TOR1AIP1 primary antibodies

  • Description

    Mouse monoclonal [RL13] to TOR1AIP1

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Full length protein corresponding to Rat TOR1AIP1. This is a Pore complex-lamina fraction isolated from rat liver nuclear envelopes.

  • Positive control

    • IHC-P: Rat lymph node, breast, colon tissue. ICC: NS-1 cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP1 antibody [RL13] (ab2737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP1 antibody [RL13] (ab2737)

    Immunohistochemical analysis (Formalin/PFA-fixed paraffin-embedded sections) of rat lymph node tissue labeling LAP1. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 at 1/50 dilution overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-LAP1 antibody [RL13] (ab2737)
    Immunocytochemistry/ Immunofluorescence - Anti-LAP1 antibody [RL13] (ab2737)

    Immunocytochemical analysis of NS-1 (Mouse myeloma cell line) cells labeling LAP1 with ab2737 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP1 antibody [RL13] (ab2737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP1 antibody [RL13] (ab2737)

    Immunohistochemical analysis (Formalin/PFA-fixed paraffin-embedded sections) rat breast tissue labeling LAP1. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 1/50 dilution overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP1 antibody [RL13] (ab2737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP1 antibody [RL13] (ab2737)

    Immunohistochemical analysis of (Formalin/PFA-fixed paraffin-embedded sections) rat colon tissue labeling LAP1. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 at 1/50 dilution overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

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