Anti-Trichohyalin antibody (ab129814)
Key features and details
- Rabbit polyclonal to Trichohyalin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Trichohyalin antibody
See all Trichohyalin primary antibodies -
Description
Rabbit polyclonal to Trichohyalin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Trichohyalin aa 1800-1900 conjugated to keyhole limpet haemocyanin.
(Peptide available asab166861) -
Positive control
- This antibody gave a positive signal in Human Skin tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications Macaque monkeyGorillaApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 210 kDa (predicted molecular weight: 253 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 210 kDa (predicted molecular weight: 253 kDa).Target
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Function
Intermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation. -
Tissue specificity
Found in the hard keratinizing tissues such as the inner root sheath (IRS) of hair follicles and medulla, and in the filiform papillae of dorsal tongue epithelium. -
Sequence similarities
Belongs to the S100-fused protein family.
Contains 2 EF-hand domains. -
Developmental stage
Expressed during late differentiation of the epidermis. -
Domain
Consists of nine domains. Domain 1 contains two EF-hand calcium-binding domains. Domains 2-4, 6, and 8 are almost entirely alpha-helical, configured as a series of peptide repeats of varying regularity, and are thought to form a single-stranded alpha-helical rod stabilized by ionic interactions. Domain 6 is the most regular and may bind KIF directly by ionic interactions. Domains 5 and 7 are less well organized and may induce folds in the molecule. Domain 9 contains the C-terminus, conserved among different species. -
Post-translational
modificationsSubstrate of transglutaminase. Some 200 arginines are probably converted to citrullines by peptidylarginine deimidase. - Information by UniProt
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Database links
- Entrez Gene: 7062 Human
- SwissProt: Q07283 Human
- Unigene: 316671 Human
- Unigene: 432416 Human
- Unigene: 553300 Human
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Alternative names
- 220K trichohyalin granular protein antibody
- Hair follicle protein AHF antibody
- MGC157889 antibody
see all
Images
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Western blot - Anti-Trichohyalin antibody (ab129814)Anti-Trichohyalin antibody (ab129814) at 1 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 253 kDa
Observed band size: 210 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa, 48 kDa, 55 kDa, 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
The predicted molecular weight of Trichohyalin is 253 kDa (SwissProt), however we expect to observe a banding pattern around 220 kDa. This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129814 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab129814 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Trichohyalin antibody (ab129814)Anti-Trichohyalin antibody (ab129814) at 1 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 253 kDa
Observed band size: 210 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa, 48 kDa, 55 kDa, 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
The predicted molecular weight of Trichohyalin is 253 kDa (SwissProt), however we expect to observe a banding pattern around 220 kDa. This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129814 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
