Antibodies, Proteins, Kits and Reagents for Life Science

Anti-LAG-3 antibody [BLR027F] (ab243884)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR027F] to LAG-3
Suitable for: WB, ICC, IHC-P, IP
Reacts with: Human

Product name

Anti-LAG-3 antibody [BLR027F]
See all LAG-3 primary antibodies
Description

Rabbit monoclonal [BLR027F] to LAG-3
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human LAG-3 aa 500-525 (C terminal). The exact sequence is proprietary. NP_002277.4 and Gene ID 3902.
Database link: P18627
General notes

This product is sold under License from Bethyl Laboratories, Inc.
This product was previously labelled as Lymphocyte Activation Gene 3.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR027F
Isotype

IgG
Research areas

Immunoprecipitation - Anti-LAG-3 antibody [BLR027F] (ab243884)

LAG-3 was immunoprecipitated from 0.5 mg HDLM-2 whole cell lysate with ab243884 at 12 µl per reaction. Western blot was performed on the immunoprecipitate using ab243884 at 1/1000 dilution.

Lane 1: ab243884 IP in HDLM-2 whole cell lysate.

Lane 2: Control IgG IP in HDLM-2 whole cell lysate.

Detection: Chemiluminescence with an exposure time of 10 seconds.
Western blot - Anti-LAG-3 antibody [BLR027F] (ab243884)

Western blot analysis using ab243884 at 1/1000 dilution.
Lane 1: HDLM-2 whole cell lysate (50 µg).
Lane 2: RPMI 8226 whole cell lysate (50 µg).
Lane3: Jurkat whole cell lysate (50 µg).
Lane 4: MJ whole cell lysate (50 µg).
Lane 5: HEK-293T whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry - Anti-LAG-3 antibody [BLR027F] (ab243884)

Formalin-fixed, paraffin-embedded HDLM-2 cells labeling LAG-3 using ab243884 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR027F] (ab243884)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 using ab243884 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Anti-LAG-3 antibody [BLR027F] (ab243884)

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