Anti-L1CAM antibody [M023] - Extracellular domain (ab253290)
![Anti-L1CAM antibody [M023] - Extracellular domain (ab253290)](https://www.abcam.com/ps/products/253/ab253290/Images/ab253290-382897-anti-l1cam-antibody-m023-extracellular-domain-elisa.jpg "Anti-L1CAM antibody [M023] - Extracellular domain (ab253290)")
Key features and details
- Mouse monoclonal [M023] to L1CAM - Extracellular domain
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-L1CAM antibody [M023] - Extracellular domain
See all L1CAM primary antibodies -
Description
Mouse monoclonal [M023] to L1CAM - Extracellular domain -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WB, ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Other Immunogen Type within Human L1CAM. The exact sequence is proprietary. Clone M023 was generated from a proprietary antigen related to the native human L1CAM expressed in the MeWo melanoma cell line
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Positive control
- WB: MeWo, MDA-MB-231, A431 and MCF7 cell lysate. Human brain tissue lysate. ICC/IF: MeWo cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
M023 -
Isotype
IgG1 -
Research areas
Images
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ELISA - Anti-L1CAM antibody [M023] - Extracellular domain (ab253290)
Representative Standard Curve using mouse monoclonal anti-L1CAM for ELISA capture of human recombinant L1CAM protein with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
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![Immunocytochemistry/ Immunofluorescence - Anti-L1CAM antibody [M023] - Extracellular domain (ab253290)](https://www.abcam.com/ps/products/253/ab253290/Images/ab253290-343901-AntiL1CAM-antibody-M023--Extracellular-domain-immunocytochemistry-mewo-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-L1CAM antibody [M023] - Extracellular domain (ab253290)Paraformaldehyde-fixed MeWo (Human malignant melanoma cell line) cells labeling L1CAM using ab253290 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.
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![Western blot - Anti-L1CAM antibody [M023] - Extracellular domain (ab253290)](https://www.abcam.com/ps/products/253/ab253290/Images/ab253290-343900-AntiL1CAM-antibody-M023--Extracellular-domain-western-blot-MeWo-MDAMB231-A431-MCF7-human-brain-tissue-lysate.jpg)
Western blot - Anti-L1CAM antibody [M023] - Extracellular domain (ab253290)All lanes : Anti-L1CAM antibody [M023] - Extracellular domain (ab253290) at 1/1000 dilution
Lane 1 : MDA-MB-231 (Human cell line) cell lysate
Lane 2 : MeWo (Human malignant melanoma cell line) cell lysate
Lane 3 : Human brain tissue lysate
Lane 4 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
Predicted band size: 140 kDaWestern blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hour at room temperature.