APC Anti-NCAM1 antibody [EPR21827] (ab237383)
![APC Anti-NCAM1 antibody [EPR21827] (ab237383)](https://www.abcam.com/ps/products/237/ab237383/Images/ab237383-391582-AntiNCAM1-antibody-EPR21827-Allophycocyanin-immunocytochemistry-neuro2a-mouse.jpg "APC Anti-NCAM1 antibody [EPR21827] (ab237383)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EPR21827] to NCAM1
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
Overview
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Product name
APC Anti-NCAM1 antibody [EPR21827]
See all NCAM1 primary antibodies -
Description
APC Rabbit monoclonal [EPR21827] to NCAM1 -
Host species
Rabbit -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseICC/IF Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Neuro2a cells. ICC/IF: Neuro2a cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21827 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - APC Anti-NCAM1 antibody [EPR21827] (ab237383)
Immunofluorescent analysis of 100% methanol-fixed Neuro2a (Mouse neuroblastoma cell line) cells labeling NCAM1 with ab237383 at 1/100 dilution (5 µg/ml), followed by ab195887 anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 488) (green). The nuclear counterstain is DAPI (blue).
Negative control: L-929 cells.
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![Flow Cytometry - APC Anti-NCAM1 antibody [EPR21827] (ab237383)](https://www.abcam.com/ps/products/237/ab237383/Images/ab237383-391581-AntiNCAM1-antibody-EPR21827-Allophycocyanin-flow-cytometry-neuro2a-mouse.jpg)
Flow Cytometry - APC Anti-NCAM1 antibody [EPR21827] (ab237383)Flow cytometric analysis of L-929 (Mouse connective tissue fibroblast cell line, Left) / Neuro2a (Mouse neuroblastoma cell line, Right) labeling NCAM1 with ab237383 at 1/500 dilution (red) compared with ab232814 Rabbit IgG, monoclonal [EPR25A] - Isotype Control - Allophycocyanin (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).
Negative control: L-929 cells.
Gated on viable cells.
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![APC Anti-NCAM1 antibody [EPR21827] (ab237383)](https://www.abcam.com/ps/products/237/ab237383/Images/ab237383-3-benefits-of-recombinant-antibodies.png)
APC Anti-NCAM1 antibody [EPR21827] (ab237383)
