Anti-Ku80 antibody (ab97433)
Key features and details
- Rabbit polyclonal to Ku80
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Ku80 antibody
See all Ku80 primary antibodies -
Description
Rabbit polyclonal to Ku80 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a sequence within amino acids 315-563 of Human Ku80 (NP_066964).
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Positive control
- WB:HEK-293T, A431, HeLa, HepG2, Neuro2a, PC-12 and Rat2 whole cell lysate. IHC: Cal27 Xenograft tissue. IF: HeLa cells. IP: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Ku80 antibody (ab97433) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 83 kDa7.5% SDS-PAGE
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Paraffin-embedded Cal27 xenograft tissue stained Ku80 using ab97433 at 1/100 dilution in immunohistochemical analysis.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Immunofluorescence analysis of 4% paraformaldehyde (RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Ku80 at nucleus at 1/500 dilution. Red: phalloidin, a cytoskeleton marker, stained by phalloidin 1/200 dilution.
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The immunoprecipitated Ku80 protein was detected byab97433 at 1/1000 dilution, followed by anti-rabbit IgG antibody.
IP Sample: 1000 μg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate.
A - 40 μg HeLa whole cell lysate.
B - Control with 2.5 μg of preimmune rabbit IgG C.
C - Immunoprecipitation of Ku80 protein by 2.5 μg of ab97433.
7.5% SDS-PAGE
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Anti-Ku80 antibody (ab97433) at 1/1000 dilution + Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 83 kDa
7.5% SDS-PAGE. -
All lanes : Anti-Ku80 antibody (ab97433) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 83 kDa7.5% SDS-PAGE