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Anti-Ku80 antibody (ab97433)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Ku80 antibody (ab97433)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Ku80
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Ku80 antibody
    See all Ku80 primary antibodies
  • Description

    Rabbit polyclonal to Ku80
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to a sequence within amino acids 315-563 of Human Ku80 (NP_066964).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB:HEK-293T, A431, HeLa, HepG2, Neuro2a, PC-12 and Rat2 whole cell lysate. IHC: Cal27 Xenograft tissue. IF: HeLa cells. IP: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Non Homol. End Joining

Images

  • Western blot - Anti-Ku80 antibody (ab97433)
    Western blot - Anti-Ku80 antibody (ab97433)
    All lanes : Anti-Ku80 antibody (ab97433) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 83 kDa



    7.5% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku80 antibody (ab97433)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku80 antibody (ab97433)

    Paraffin-embedded Cal27 xenograft tissue stained Ku80 using ab97433 at 1/100 dilution in immunohistochemical analysis.

    Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

  • Immunocytochemistry/ Immunofluorescence - Anti-Ku80 antibody (ab97433)
    Immunocytochemistry/ Immunofluorescence - Anti-Ku80 antibody (ab97433)

    Immunofluorescence analysis of 4% paraformaldehyde (RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Ku80 at nucleus at 1/500 dilution. Red: phalloidin, a cytoskeleton marker, stained by phalloidin 1/200 dilution.

  • Immunoprecipitation - Anti-Ku80 antibody (ab97433)
    Immunoprecipitation - Anti-Ku80 antibody (ab97433)

    The immunoprecipitated Ku80 protein was detected byab97433 at 1/1000 dilution, followed by anti-rabbit IgG antibody.

    IP Sample: 1000 μg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate.

    A -  40 μg HeLa whole cell lysate.

    B - Control with 2.5 μg of preimmune rabbit IgG C.

    C - Immunoprecipitation of Ku80 protein by 2.5 μg of ab97433.

    7.5% SDS-PAGE

     

  • Western blot - Anti-Ku80 antibody (ab97433)
    Western blot - Anti-Ku80 antibody (ab97433)
    Anti-Ku80 antibody (ab97433) at 1/1000 dilution + Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 83 kDa



    7.5% SDS-PAGE.
  • Western blot - Anti-Ku80 antibody (ab97433)
    Western blot - Anti-Ku80 antibody (ab97433)
    All lanes : Anti-Ku80 antibody (ab97433) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 83 kDa



    7.5% SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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