Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KRAS antibody [EPR23474-76] (ab275876)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23474-76] to KRAS
  • Suitable for: IP, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-KRAS antibody [EPR23474-76]
    See all KRAS primary antibodies

  • Description

    Rabbit monoclonal [EPR23474-76] to KRAS

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain tissue lysate; HeLa whole cell lysate; His-tagged human KRAS recombinant protein. Flow cyt: HeLa cells. IP: Human brain tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-KRAS antibody [EPR23474-76] (ab275876)
    Western blot - Anti-KRAS antibody [EPR23474-76] (ab275876)
    All lanes : Anti-KRAS antibody [EPR23474-76] (ab275876) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 22 kDa
    Observed band size: 21 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

     

  • Flow Cytometry - Anti-KRAS antibody [EPR23474-76] (ab275876)
    Flow Cytometry - Anti-KRAS antibody [EPR23474-76] (ab275876)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling KRAS with ab275876 at 1/50 dilution (1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730)  isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-KRAS antibody [EPR23474-76] (ab275876)
    Immunoprecipitation - Anti-KRAS antibody [EPR23474-76] (ab275876)

    KRAS was immunoprecipitated from 0.35 mg Human brain tissue lysate with ab275876 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275876 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human brain tissue lysate 10 ug

    Lane 2: ab275876 IP in Human brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275876 in human brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Western blot - Anti-KRAS antibody [EPR23474-76] (ab275876)
    Western blot - Anti-KRAS antibody [EPR23474-76] (ab275876)
    All lanes : Anti-KRAS antibody [EPR23474-76] (ab275876) at 1/1000 dilution

    Lane 1 : His-tagged human KRAS recombinant protein, 10 ng
    Lane 2 : His-tagged human HRAS recombinant protein (aa 1-186), 20 ng
    Lane 3 : His-tagged human NRAS recombinant protein (aa 1-186), 20 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 22 kDa
    Observed band size: 21 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Anti-KRAS antibody [EPR23474-76] (ab275876)
    Anti-KRAS antibody [EPR23474-76] (ab275876)

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