Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

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Anti-KRAS antibody [EPR23474-76] (ab275876)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23474-76] to KRAS
Suitable for: IP, Flow Cyt, WB
Reacts with: Human

Product name

Anti-KRAS antibody [EPR23474-76]
See all KRAS primary antibodies
Description

Rabbit monoclonal [EPR23474-76] to KRAS
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human brain tissue lysate; HeLa whole cell lysate; His-tagged human KRAS recombinant protein. Flow cyt: HeLa cells. IP: Human brain tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23474-76
Isotype

IgG
Research areas

Western blot - Anti-KRAS antibody [EPR23474-76] (ab275876)

All lanes : Anti-KRAS antibody [EPR23474-76] (ab275876) at 1/1000 dilution

Lane 1 : Human brain tissue lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

Predicted band size: 22 kDa
Observed band size: 21 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes
Flow Cytometry - Anti-KRAS antibody [EPR23474-76] (ab275876)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling KRAS with ab275876 at 1/50 dilution (1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-KRAS antibody [EPR23474-76] (ab275876)

KRAS was immunoprecipitated from 0.35 mg Human brain tissue lysate with ab275876 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275876 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Human brain tissue lysate 10 ug

Lane 2: ab275876 IP in Human brain tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275876 in human brain tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-KRAS antibody [EPR23474-76] (ab275876)

All lanes : Anti-KRAS antibody [EPR23474-76] (ab275876) at 1/1000 dilution

Lane 1 : His-tagged human KRAS recombinant protein, 10 ng
Lane 2 : His-tagged human HRAS recombinant protein (aa 1-186), 20 ng
Lane 3 : His-tagged human NRAS recombinant protein (aa 1-186), 20 ng

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 22 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-KRAS antibody [EPR23474-76] (ab275876)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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