Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)
![Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)](https://www.abcam.com/ps/products/272/ab272075/Images/ab272075-2-ab243878334301antiki67antibodyblr021eimmunohistochemistry.jpg "Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR021E] to Ki67 - BSA free
- Suitable for: WB, ICC, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Ki67 antibody [BLR021E] - BSA free
See all Ki67 primary antibodies -
Description
Rabbit monoclonal [BLR021E] to Ki67 - BSA free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P Human
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Immunogen
Synthetic peptide within Human Ki67 aa 1200-1250. The exact sequence is proprietary. Corresponds to the repetitive 66 bp element. NP_002408.2 and Gene ID 4288.
Database link: P46013 -
Positive control
- IHC-P: Human tonsil tissue. ICC/IF: OVCAR-8 cells
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
ab272075 is the carrier-free version of ab243878. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR021E -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)Formalin-fixed, paraffin-embedded human tonsil tissue stained for Ki67 using ab243878 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243878).
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![Immunocytochemistry - Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)](https://www.abcam.com/ps/products/272/ab272075/Images/ab272075-1-ab243878334302antiki67antibodyblr021eimmunocytochemistry.jpg)
Immunocytochemistry - Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)Paraffin-embedded, Formaldehyde-fixed OVCAR-8 cells labeling Ki67 using ab243878 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB stainingThis data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243878).
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![Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)](https://www.abcam.com/ps/products/272/ab272075/Images/ab272075-3-benefits-of-recombinant-antibodies.png)
Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)
