Anti-KACL antibody (ab76711)
Key features and details
- Rabbit polyclonal to KACL
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KACL antibody -
Description
Rabbit polyclonal to KACL -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human KACL aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab103565) -
Positive control
- This antibody gave a positive signal in the following lysates: Human Spleen Tissue; Human Thymus Tissue; Human Small Intestine Tissue; Human Bone Marrow Tissue; Raji Whole Cell; Jurkat Whole Cell; MOLT4 Whole Cell.
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General notes
Previously labelled as CLEC2A.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 20 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 20 kDa).Target
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Function
Plays a role in modulating the extent of T-cell expansion. Enhances the expansion of TCR-stimulated T cells by increasing their survival through enhanced expression of anti-apoptotic proteins. May modulate the capacity of T-cells to home to lymph nodes through SELL. Facilitates dedicated immune recognition of keratinocytes via interaction with its receptor KLRF2 by stimulating natural killer cell mediated cytotoxicity. -
Tissue specificity
Mainly expressed in skin. Also expressed in keratinocytes, spleen, thymus, small intestine, peripheral blood monocytes, bone marrow, ovary, testis and skin. High expression in CD8(+), B lymphocytes and naive CD4(+) T cells. Restricted mostly to proliferating lymphocytes. Not detected in myeloid leukocytes or natural killer (NK) cells. -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 387836 Human
- Omim: 612087 Human
- SwissProt: Q6UVW9 Human
- Unigene: 1087866 Human
- Unigene: 527665 Human
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Alternative names
- C-type lectin domain family 2 member A antibody
- CLC2A_HUMAN antibody
- CLEC2A antibody
see all
Images
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Western blot - Anti-KACL antibody (ab76711)All lanes : Anti-KACL antibody (ab76711) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Human thymus tissue lysate - total protein (ab30146)
Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
Lane 4 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 7 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Additional bands at: 300 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesKACL contains a potential glycosylation site (SwissProt), which may explain its migration at a higher molecular weight than predicted. The band at 32-kDa is consistent with what has been reported in the literature (PMID:16174766).
Datasheets and documents
References (0)
ab76711 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-KACL antibody (ab76711)All lanes : Anti-KACL antibody (ab76711) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Human thymus tissue lysate - total protein (ab30146)
Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
Lane 4 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 7 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Additional bands at: 300 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesKACL contains a potential glycosylation site (SwissProt), which may explain its migration at a higher molecular weight than predicted. The band at 32-kDa is consistent with what has been reported in the literature (PMID:16174766).