Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14220] to TPD52 - BSA and Azide free
  • Suitable for: IHC-P, ICC, IP, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TPD52 antibody [EPR14220] - BSA and Azide free
    See all TPD52 primary antibodies

  • Description

    Rabbit monoclonal [EPR14220] to TPD52 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Based on immunogen sequence analysis, this antibody recognizes 4 isoforms with the predicted MWs of 24KDa, 20KDa, 26 KDa and 22KDa, respectively.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250629 is the carrier-free version of ab182578. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250629 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14220

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
    Western blot - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
    All lanes : Anti-TPD52 antibody [EPR14220] (ab182578) at 1/20000 dilution

    Lane 1 : HCT-116 cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : THP1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa



    This data was developed using ab182578, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)

    This data was developed using ab182578, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling TPD52 using ab182578 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
    Immunocytochemistry - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
    This data was developed using ab182578, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of MCF7 cells labeling TPD52 using ab182578 at 1/500 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.
  • Flow Cytometry - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
    Flow Cytometry - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
    This data was developed using ab182578, the same antibody clone in a different buffer formulation.Flow cytometry analysis of Raji (human Burkitt's lymphoma) cells labeling TPD52 (red) with ab182578 at a 1/350 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
  • Immunoprecipitation - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
    Immunoprecipitation - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
    This data was developed using ab182578, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from HCT-116 cell lysate (+) or a negative control (-) immunoprecipitated using ab182578 at 1/50 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
  • Western blot - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
    Western blot - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
    Anti-TPD52 antibody [EPR14220] (ab182578) at 1/10000 dilution + SW480 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa



    This data was developed using ab182578, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
    This data was developed using ab182578, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human Prostatic hyperplasia tissue labeling TPD52 using ab182578 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
    Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)

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