Recombinant human AKT1 protein (Active) (ab62279)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human AKT1 protein (Active)
See all AKT1 proteins and peptides -
Biological activity
124 nmol/min/mg
124 nmol phosphate incorporated into Akt substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP and total of 0.83 µCi/µl P-32.
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Purity
> 90 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSDVAIVKEG WLHKRGEYIK TWRPRYFLLK NDGTFIGYKE RPQDVDQREA PLNNFSVAQC QLMKTERPRP NTFIIRCLQW TTVIERTFHV ETPEEREEWT TAIQTVADGL KKQEEEEMDF RSGSPSDNSG AEEMEVSLAK PKHRVTMNEF EYLKLLGKGT FGKVILVKEK ATGRYYAMKI LKKEVIVAKD EVAHTLTENR VLQNSRHPFL TALKYSFQTH DRLCFVMEYA NGGELFFHLS RERVFSEDRA RFYGAEIVSA LDYLHSEKNV VYRDLKLENL MLDKDGHIKI TDFGLCKEGI KDGATMKTFC GTPEYLAPEV LEDNDYGRAV DWWGLGVVMY EMMCGRLPFY NQDHEKLFEL ILMEEIRFPR TLGPEAKSLL SGLLKKDPKQ RLGGGSEDAK EIMQHRFFAG IVWQHVYEKK LSPPFKPQVT SETDTRYFDE EFTAQMITIT PPDQDDSMEC VDSERRPHFP QFSYSASGTA -
Predicted molecular weight
85 kDa including tags -
Amino acids
1 to 480 -
Tags
GST tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Western blot - Recombinant human AKT1 protein (ab62279)
Primary Antibodies: Lane 1: Anti Akt-1 antibody (ab54752) at 5µg/mL dilution. Lane 2: Anti Akt-1 antibody (ab81283) at 1/5000 dilution. Sample: Akt-1 recombinant protein (Tagged)(ab62279) 50ng. Secondary Antibody: Lane 1: Goat polyclonal to Mouse IgG H&L Pre-Adsorbed (HRP) at 1:5000 developed using the ECL technique. Lane 2: Goat polyclonal to Rabbit IgG H&L - Pre-Adsorbed (HRP) at 1:5000 developed using the ECL technique. Performed under reducing conditions. Predicted band size : 80kDa. Observed band size : 80kDa. Blocking step: 5% Milk in 50mM TBS+0.05% Tween for 1 hour at RT. Primary antibody buffer: 5% BSA in 50mM TBS+0.05% Tween overnight at 4°C. Secondary antibody buffer: 10% blocking ab126587 in water for 2 hours at RT. Exposure time : 5 minutes
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Functional Studies - Recombinant human AKT1 protein (ab62279)ab62279 Kinase activity plot. -
SDS-PAGE - Recombinant human AKT1 protein (ab62279)ab62279 on SDS-PAGE, stained with Coomassie Blue.
Band at ~85kDa.
