Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AKT3 + AKT1 antibody [E45] - BSA and Azide free (ab227994)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E45] to AKT3 + AKT1 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-AKT3 + AKT1 antibody [E45] - BSA and Azide free

  • Description

    Rabbit monoclonal [E45] to AKT3 + AKT1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Squamous lung carcinoma ICC/IF: SK-BR-3 cells

  • General notes

    ab227994 is the carrier-free version of ab32038.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-AKT3 + AKT1 antibody [E45] - BSA and Azide free (ab227994)
    Immunocytochemistry/ Immunofluorescence - Anti-AKT3 + AKT1 antibody [E45] - BSA and Azide free (ab227994)

    Immunocytochemistry/ Immunofluorescence analysis of SK-BR-3 (human breast adenocarcinoma epithelial cell) cells labeling AKT3+AKT1 with purified ab32038 at 1/500 dilution (2.1 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32038).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 + AKT1 antibody [E45] - BSA and Azide free (ab227994)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 + AKT1 antibody [E45] - BSA and Azide free (ab227994)

    This IHC data was generated using the same anti-AKT1/3 antibody clone, E45, in a different buffer formulation (cat# ab32038).

    Immunohistochemistry (Paraffin-embedded sections) analysis using ab32038 at a dilution of 1/1000 - 1/1250 and squamous lung carcinoma.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-AKT3 + AKT1 antibody [E45] - BSA and Azide free (ab227994)
    Anti-AKT3 + AKT1 antibody [E45] - BSA and Azide free (ab227994)

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