Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Insulin Receptor beta antibody [EPR23566-103]
See all Insulin Receptor beta primary antibodies
Description

Rabbit monoclonal [EPR23566-103] to Insulin Receptor beta
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MDA-MB-231, PC-3, HepG2, LNCaP and C2C12 whole cell lysate; Human liver and kidney tissue lysate; Mouse liver and skeletal muscle tissue lysate; Rat liver tissue lysate; AR42J whole cell lysate; Rat-1 whole cell lysate; Recombinant mouse INSR active protein (aa28-748 & aa753-946). IHC-P: Human liver tissue. Human kidney cancer tissue; Mouse and rat liver tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23566-103
Isotype

IgG
Research areas

Western blot - Anti-Insulin Receptor beta antibody [EPR23566-103] (ab278100)

All lanes : Anti-Insulin Receptor beta antibody [EPR23566-103] (ab278100) at 1/1000 dilution

Lane 1 : T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Observed band size: 45,95,210 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Bands of 95KDa and 45KDa respectively represent full length and the proteolytic cleavage fragment (β') of insulin receptor beta subunit.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:19017805, 20655470, 2859121, 1617668, 29099049).

Low expression: T-47D (PMID: 1617668, 29099049)

Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor beta antibody [EPR23566-103] (ab278100)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Insulin Receptor beta with ab278100 at 1/2000 (0.24 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human liver. The section was incubated with ab278100 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-Insulin Receptor beta antibody [EPR23566-103] (ab278100)

All lanes : Anti-Insulin Receptor beta antibody [EPR23566-103] (ab278100) at 1/1000 dilution

Lane 1 : Human liver tissue lysate
Lane 2 : Human kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Observed band size: 45,95 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Bands of 95KDa and 45KDa respectively represent full length and the proteolytic cleavage fragment (β') of insulin receptor beta subunit.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:19017805, 20655470, 2859121, 1617668, 29099049).

Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor beta antibody [EPR23566-103] (ab278100)

Immunohistochemical analysis of paraffin-embedded human kidney cancer tissue labeling Insulin Receptor beta with ab278100 at 1/2000 (0.24 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Mainly positive staining on endothelial cells of human kidney cancer (PMID:20182859). The section was incubated with ab278100 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-Insulin Receptor beta antibody [EPR23566-103] (ab278100)

All lanes : Anti-Insulin Receptor beta antibody [EPR23566-103] (ab278100) at 1/1000 dilution

Lane 1 : LNCaP (human prostate carcinoma epithelial cell), whole cell lysate
Lane 2 : LNCaP (human prostate carcinoma epithelial cell) treated with PNGase F whole cell lysate
Lane 3 : C2C12 (mouse myoblasts myoblast), whole cell lysate
Lane 4 : C2C12 (mouse myoblasts myoblast) treated with PNGase F whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Observed band size: 45,95,210 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Bands of 95KDa and 45KDa respectively represent full length and the proteolytic cleavage fragment (β') of insulin receptor beta subunit.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:19017805, 20655470, 2859121, 1617668, 29099049).

This blot was developed using a higher sensitivity ECL substrate.

Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor beta antibody [EPR23566-103] (ab278100)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Insulin Receptor beta with ab278100 at 1/2000 (0.24 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse liver. The section was incubated with ab278100 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-Insulin Receptor beta antibody [EPR23566-103] (ab278100)

All lanes : Anti-Insulin Receptor beta antibody [EPR23566-103] (ab278100) at 1/1000 dilution

Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Observed band size: 45,95,210 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Bands of 95KDa and 45KDa respectively represent full length and the proteolytic cleavage fragment (β') of insulin receptor beta subunit.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:19017805, 20655470, 2859121, 1617668, 29099049).

This blot was developed using a higher sensitivity ECL substrate.

Exposure time: 3 minutes
Western blot - Anti-Insulin Receptor beta antibody [EPR23566-103] (ab278100)

All lanes : Anti-Insulin Receptor beta antibody [EPR23566-103] (ab278100) at 1/1000 dilution

Lane 1 : Rat liver tissue lysate
Lane 2 : AR42J (rat pancreatic tumor epithelial cell), whole cell lysate
Lane 3 : Rat-1 (rat embryonic fibroblast), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Observed band size: 45,95,210 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Bands of 95KDa and 45KDa respectively represent full length and the proteolytic cleavage fragment (β') of insulin receptor beta subunit.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:19017805, 20655470, 2859121, 1617668, 29099049).

This blot was developed using a higher sensitivity ECL substrate.

Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor beta antibody [EPR23566-103] (ab278100)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Insulin Receptor beta with ab278100 at 1/2000 (0.24 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat liver. The section was incubated with ab278100 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-Insulin Receptor beta antibody [EPR23566-103] (ab278100)

Anti-Insulin Receptor beta antibody [EPR23566-103] (ab278100) at 1/1000 dilution + Recombinant mouse INSR active protein (aa 28-748 & aa 753-946), 20ng

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Observed band size: 150,82 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

This blot was developed using a higher sensitivity ECL substrate.

Exposure time: 3 minutes