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Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)

Key features and details

  • Mouse monoclonal [C43] to Influenza A Virus Nucleoprotein
  • Suitable for: WB, ELISA, ICC/IF
  • Reacts with: Influenza A
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Influenza A Virus Nucleoprotein antibody [C43]
    See all Influenza A Virus Nucleoprotein primary antibodies

  • Description

    Mouse monoclonal [C43] to Influenza A Virus Nucleoprotein

  • Host species

    Mouse

  • Specificity

    Reacts with NP of all influenza A viruses so far tested, including seasonal H2N2, H3N2(A/Sydney/5/1997), and H5N1(A/crow/Kyoto53/2004), H5N1 (A/duck/Egypt/D2br10/07), H5N1(A/duck/HK/342/78), H5N2(A/crow/Kyoto/53/04), H9N1, H9N2 (A/Turkey/Wisconsin/1/66) and H1N1 (seasonal: A/New Caledonia/20/99. Pandemic: A/Suita/01/2009 and swine: A/PuertoRico/8/34).

    No cross reactivity with influenza B viruses.

  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details

  • Species reactivity

    Reacts with: Influenza A

  • Immunogen

    Tissue, cells or virus corresponding to Influenza A Virus Nucleoprotein. Human Influenza A Virus H3N2 strain
    Database link: P69291

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 6
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...

  • Purity

    Proprietary Purification

  • Purification notes

    Produced in serum-free medium and purified by proprietary chromatography procedure under mild conditions. 90~95% pure by SDS-PAGE

  • Clonality

    Monoclonal

  • Clone number

    C43

  • Isotype

    IgG2a

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
    Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
    Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were taken at 3, 9, and 24 hours post-infection. Influenza A Virus Nucleoprotein antibody (ab128193) efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an green flurescent secondary antibody.
  • Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
    Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
    Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/1000 dilution + MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34

    Secondary
    Rabbit Anti-Mouse IgG H&L (HRP) (ab97046) at 1/10000 dilution
  • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
    Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
    Immunofluorescence assay of 293T cells expressing HA or NP of pandemic (H1N1) 2009 influenza A virus (A/Suita/1/2009). Influenza A Virus Nucleoprotein antibody (ab128193) specifically recognized NP-expressing cells while a proprietary mouse anti-HA monoclonal antibody specifically recognized HA.
  • Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
    Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
    All lanes : Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/300 dilution

    Lanes 1 & 6 & 11 : MDCK cells mock infected
    Lane 2 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 3 hrs post-infection
    Lane 3 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 9 hrs post-infection
    Lane 4 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 24 hrs post-infection
    Lane 5 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 48 hrs post-infection
    Lane 7 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 3 hrs post-infection
    Lane 8 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 9 hrs post-infection
    Lane 9 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 24 hrs post-infection
    Lane 10 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 48 hrs post-infection
    Lane 12 : MDCK cells infected H5N1
    (A/duck/HK/342/78)collected 3 hrs post-infection
    Lane 13 : MDCK cells infected with H5N1
    (A/duck/HK/342/78) collected 9 hrs post-infection
    Lane 14 : MDCK cells infected with H5N1
    (A/duck/HK/342/78) collected 24 hrs post-infection
    Lane 15 : MDCK cells infected with H5N1
    (A/duck/HK/342/78) collected 48 hrs post-infection


    Tubulin was used as loading control.

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