Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-5RA antibody [CAL40] - BSA and Azide free (ab251603)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL40] to IL-5RA - BSA and Azide free
  • Suitable for: ELISA, IHC-P
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-IL-5RA antibody [CAL40] - BSA and Azide free
    See all IL-5RA primary antibodies

  • Description

    Rabbit monoclonal [CAL40] to IL-5RA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IP or WB

  • Species reactivity

    Reacts with: Human, Recombinant fragment

  • Immunogen

    Synthetic peptide within Human IL-5RA aa 1-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q01344

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human tonsil tissue.

  • General notes

    Ab251603 is the carrier-free version of ab237716. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251603 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purity is greater than 99%.

  • Clonality

    Monoclonal

  • Clone number

    CAL40

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-5RA antibody [CAL40] - BSA and Azide free (ab251603)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-5RA antibody [CAL40] - BSA and Azide free (ab251603)

    Immunohistochemical analysis of paraffin-embedded human tonsil  tissue labeling IL-5RA with ab237716 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human tonsil is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    The section was incubated with ab237716 for 15 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237716).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-5RA antibody [CAL40] - BSA and Azide free (ab251603)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-5RA antibody [CAL40] - BSA and Azide free (ab251603)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for IL5RA using ab237716 at 0.125 μg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237716).

  • ELISA - Anti-IL-5RA antibody [CAL40] - BSA and Azide free (ab251603)
    ELISA - Anti-IL-5RA antibody [CAL40] - BSA and Azide free (ab251603)

    This data was developed using ab237716, the same antibody clone in a different buffer formulation.

    ELISA analysis of Human IL5RA recombinant protein at 1000 ng/mL with ab237716. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Anti-IL-5RA antibody [CAL40] - BSA and Azide free (ab251603)
    Anti-IL-5RA antibody [CAL40] - BSA and Azide free (ab251603)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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