Anti-IL-4R (phospho Y497) antibody (ab61099)
Key features and details
- Rabbit polyclonal to IL-4R (phospho Y497)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-4R (phospho Y497) antibody
See all IL-4R primary antibodies -
Description
Rabbit polyclonal to IL-4R (phospho Y497) -
Host species
Rabbit -
Specificity
Detects endogenous levels of IL4R only when phosphorylated at tyrosine 497 (corresponding to mouse tyrosine 500) -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human IL-4R.
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Positive control
- 293 and HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography, using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IL-4R (phospho Y497) antibody (ab61099)All lanes : Anti-IL-4R (phospho Y497) antibody (ab61099) at 1/500 dilution
Lane 1 : Extracts from 293 cells
Lane 2 : Extracts from 293 cells with immunizing phosphopeptide
Predicted band size: 89 kDa
Observed band size: 89 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-IL-4R (phospho Y497) antibody (ab61099)ICC/IF image of ab61099 (at a 1/500 dilution) stained HeLa cells, minus immunising peptide (left image) and plus immunising peptide (right image).
