Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling IL4R with ab203398 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Flow Cytometry analysis of Raji cells labeling IL-4R with ab203398. Cells were fixed with 2% PFA (10min at room temperature). The cells were then incubated in 1 X PBS containing 0.5% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Staining with ab203398 was performed at 1/100 for 30 min at room temperature. The secondary antibody, Goat anti-rabbit IgG-PE, was used at a 1/200 dilution for 40 min at room temperature. Primary antibody staining (green) is compared to compared to unstained cells (dark blue), secondary only (light blue), and isotype control (orange).

Immunocytochemistry/ Immunofluorescence analys of A431 cells labeling IL-4R with ab203398 1/100 for 90 minutes at 37°C. Cells were fixed with 4% Paraformaldehyde, Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min. Secondary antibody was Goat Anti-Rabbit IgG antibody with FITC conjugation at 37°C for 90 minutes, DAPI (5ug/ml, blue) was used to stain the cell nuclei.

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for IL4R using ab203398 at 1/200 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, AF488 conjugated at 1/200 dilution.