Anti-IL-26 antibody [EPR22268-141] - BSA and Azide free (ab254476)
![Anti-IL-26 antibody [EPR22268-141] - BSA and Azide free (ab254476)](https://www.abcam.com/ps/products/254/ab254476/Images/ab254476-3-224198IHC1.jpg "Anti-IL-26 antibody [EPR22268-141] - BSA and Azide free (ab254476)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22268-141] to IL-26 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Overview
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Product name
Anti-IL-26 antibody [EPR22268-141] - BSA and Azide free
See all IL-26 primary antibodies -
Description
Rabbit monoclonal [EPR22268-141] to IL-26 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: THP-1 whole cell lysate; His-tagged human IL-26 recombinant protein (aa 22-171). IP: THP-1 treated with 80nM PMA (Phorbol-12-myristate-13-acetate) overnight, then 100ng/ml LPS (lipopolysaccharide) for 3h and add 300ng/ml BFA (Brefeldin A) for 3h, whole cell lysate. IHC-P: Human colon and cervical carcinoma tissue. ICC/IF: THP-1 cells treated with 80nM PMA (Phorbol-12-myristate-13-acetate) overnight, then 100ng/ml LPS (lipopolysaccharide) for 3h and add 300ng/ml BFA (Brefeldin A) for 3h.
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General notes
Ab254476 is the carrier-free version of ab224198. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab254476 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22268-141 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-26 antibody [EPR22268-141] - BSA and Azide free (ab254476)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling IL-26 with ab224198 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on lymphocytes of human colon (PMID: 18483078, 28852311) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. The section was incubated with ab224198 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224198).
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![Immunocytochemistry/ Immunofluorescence - Anti-IL-26 antibody [EPR22268-141] - BSA and Azide free (ab254476)](https://www.abcam.com/ps/products/254/ab254476/Images/ab254476-1-224198ICC1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IL-26 antibody [EPR22268-141] - BSA and Azide free (ab254476)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) cells labeling IL-26 with ab224198 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in THP-1 cell line treated with 80nM PMA (Phorbol-12-myristate-13-acetate) for 16h, then 100ng/ml LPS (lipopolysaccharide) for 3h and add 300ng/ml BFA (Brefeldin A) for 3h. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Cells were either untreated, or treated with 80nM PMA (Phorbol-12-myristate-13-acetate) for 16h, then 100ng/ml LPS (lipopolysaccharide) for 3h and add 300ng/ml BFA (Brefeldin A) for 3h.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224198).
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![Immunoprecipitation - Anti-IL-26 antibody [EPR22268-141] - BSA and Azide free (ab254476)](https://www.abcam.com/ps/products/254/ab254476/Images/ab254476-2-224198IP1.jpg)
Immunoprecipitation - Anti-IL-26 antibody [EPR22268-141] - BSA and Azide free (ab254476)IL-26 was immunoprecipitated from 0.35 mg of THP-1 (human monocytic leukemia cell line) (treated with 80nM PMA (Phorbol-12-myristate-13-acetate) overnight, then 100ng/ml LPS (lipopolysaccharide) for 3h and add 300ng/ml BFA (Brefeldin A) for 3h) whole cell lysate with ab224198 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224198 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.
Lane 1: THP-1 (treated as above) whole cell lysate 10 μg (Input).
Lane 2: ab224198 IP in THP-1 (treated as above) whole cell lysate.
Lane 3: : Rabbit monoclonal IgG (ab172730) instead of ab224198 in THP-1 (treated as above) whole cell lysate.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 mins.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224198).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-26 antibody [EPR22268-141] - BSA and Azide free (ab254476)](https://www.abcam.com/ps/products/254/ab254476/Images/ab254476-4-224198IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-26 antibody [EPR22268-141] - BSA and Azide free (ab254476)Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labeling IL-26 with ab224198 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on lymphocytes of human cervical carcinoma (PMID: 18483078, 28852311) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. The section was incubated with ab224198 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224198).
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![Anti-IL-26 antibody [EPR22268-141] - BSA and Azide free (ab254476)](https://www.abcam.com/ps/products/254/ab254476/Images/ab254476-5-benefits-of-recombinant-antibodies.png)
Anti-IL-26 antibody [EPR22268-141] - BSA and Azide free (ab254476)
