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Cancer Tumor biomarkers Receptors

Human FCGRT (FCRN) knockout HEK-293T cell lysate (ab257435)

Price and availability

331 689 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Human FCGRT (FCRN) knockout HEK-293T cell lysate (ab257435)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

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Overview

  • Product name

    Human FCGRT (FCRN) knockout HEK-293T cell lysate
    See all FCGRT/FCRN kits
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HEK293T
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 3.
  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab260237 - Human FCGRT knockout HEK293T cell lysate 1 x 100µg
    ab255594 - Human wild-type HEK293T cell lysate 1 x 100µg
  • Research areas

    • Immunology
    • Immunoglobulins
    • Receptors
    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Immunology
    • Adaptive Immunity
    • MHC
    • Class I
  • Cell type

    epithelial
  • STR Analysis

    Amelogenin X D5S818: 8, 9 D13S317: 11, 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 15, 20 TH01: 7, 9.3 TPOX: 11, 12 CSF1PO: 12

Target

  • Function

    Binds to the Fc region of monomeric immunoglobulins gamma. Mediates the uptake of IgG from milk. Possible role in transfer of immunoglobulin G from mother to fetus.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P55899 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Alpha chain
    • Fc fragment of IgG, receptor transporter, alpha
    • FCGRN_HUMAN
    • FCGRT
    • FcRn
    • FcRn alpha chain
    • FCRN, alpha chain
    • IgG Fc fragment receptor transporter alpha chain
    • IgG Gc receptor
    • IgG receptor FcRn large subunit p51
    • IgG receptor FcRn large subunit p51 precursor
    • Immunoglobulin receptor, intestinal, heavy chain
    • Neonatal Fc receptor
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab260237 - Human FCGRT knockout HEK293T cell lysate 1 x 100µg
    ab255594 - Human wild-type HEK293T cell lysate 1 x 100µg
  • Research areas

    • Immunology
    • Immunoglobulins
    • Receptors
    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Immunology
    • Adaptive Immunity
    • MHC
    • Class I
  • Cell type

    epithelial
  • STR Analysis

    Amelogenin X D5S818: 8, 9 D13S317: 11, 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 15, 20 TH01: 7, 9.3 TPOX: 11, 12 CSF1PO: 12

Images

  • Western blot - Human FCGRT knockout HEK293T cell lysate (ab257435)
    Western blot - Human FCGRT knockout HEK293T cell lysate (ab257435)

    Lane 1: Wild-type A549 cell lysate (20 µg)

    Lane 2: FCGRT knockout A549 cell lysate (20 µg)

    Lane 3: HEK293 cell lysate (20 µg)

    Lane 4: Lncap cell lysate (20 µg)

    Lanes 1-4: Merged signal (red and green). Green - ab228975 observed at 40 kDa. Red - loading control ab7291 observed at 50 kDa.

    ab228975 Anti-FCGRT/FCRN antibody [EPR22627-25] was shown to specifically react with FCGRT/FCRN in wild-type A549 cells. Loss of signal was observed when knockout cell line ab265075 (knockout cell lysate ab257435) was used. Wild-type and FCGRT/FCRN knockout samples were subjected to SDS-PAGE. ab228975 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human FCGRT knockout HEK293T cell lysate (ab257435)
    Sanger Sequencing - Human FCGRT knockout HEK293T cell lysate (ab257435)
    Homozygous: 14 bp deletion in exon 3

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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