Anti-IL-1RAPL1 antibody (ab117480)
Key features and details
- Rabbit polyclonal to IL-1RAPL1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-IL-1RAPL1 antibody
See all IL-1RAPL1 primary antibodies -
Description
Rabbit polyclonal to IL-1RAPL1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in Mouse Brain and Mouse Hippocampus tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications ChickenHumanChimpanzeeMacaque monkeyGorillaChinese hamsterOrangutanApplication Abreviews Notes WB 1/250. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa).Notes WB
1/250. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa).Target
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Function
May regulate secretion and presynaptic differentiation through inhibition of the activity of N-type voltage-gated calcium channel. May activate the MAP kinase JNK. -
Tissue specificity
Detected at low levels in heart, skeletal muscle, ovary, skin, amygdala, caudate nucleus, corpus callosum, hippocampus, substantia nigra and thalamus. Detected at very low levels in tonsil, prostate, testis, small intestine, placenta, colon and fetal liver. -
Involvement in disease
Defects in IL1RAPL1 are the cause of mental retardation X-linked type 21 (MRX21) [MIM:300143]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Cell membrane. Cytoplasm. May localize to the cell body and growth cones of dendrite-like processes. - Information by UniProt
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Database links
- Entrez Gene: 11141 Human
- Entrez Gene: 331461 Mouse
- Omim: 300206 Human
- SwissProt: Q9NZN1 Human
- SwissProt: P59823 Mouse
- Unigene: 658912 Human
- Unigene: 121680 Mouse
- Unigene: 426143 Mouse
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Alternative names
- IL 1 RAPL 1 antibody
- IL 1RAPL 1 antibody
- IL-1-RAPL-1 antibody
see all
Images
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Western blot - Anti-IL-1RAPL1 antibody (ab117480)All lanes : Anti-IL-1RAPL1 antibody (ab117480) at 1/250 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Mouse Hippocampus Tissue Lysate
Lane 3 : Brain (Mouse) Tissue Lysate with Immunising peptide at 1/250 dilution
Lane 4 : Mouse Hippocampus Tissue Lysate with Immunising peptide at 1/250 dilution
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 103 kDa (possible non-specific binding), 32 kDa (possible non-specific binding)
Exposure time: 12 minutesIL1RAPL1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab117480 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab117480 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-IL-1RAPL1 antibody (ab117480)All lanes : Anti-IL-1RAPL1 antibody (ab117480) at 1/250 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Mouse Hippocampus Tissue Lysate
Lane 3 : Brain (Mouse) Tissue Lysate with Immunising peptide at 1/250 dilution
Lane 4 : Mouse Hippocampus Tissue Lysate with Immunising peptide at 1/250 dilution
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 103 kDa (possible non-specific binding), 32 kDa (possible non-specific binding)
Exposure time: 12 minutesIL1RAPL1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab117480 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
