Anti-IL-17A antibody [4K5F6] (ab189377)
![Anti-IL-17A antibody [4K5F6] (ab189377)](https://www.abcam.com/ps/products/189/ab189377/Images/ab189377-224031-anti-il-17a-antibody-4k5f6-immunohistochemistry.jpg "Anti-IL-17A antibody [4K5F6] (ab189377)")
Key features and details
- Mouse monoclonal [4K5F6] to IL-17A
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-IL-17A antibody [4K5F6]
See all IL-17A primary antibodies -
Description
Mouse monoclonal [4K5F6] to IL-17A -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human IL-17A aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_002181.1).
Database link: Q16552 -
Positive control
- Human small intestine tissue; Human lymphocytes stimulated with PMA (5 ng/ml), Ionomycin (1 mM), and Brefeldin A (1X) for 5 hrs; Human full-length recombinant IL-17A protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4K5F6 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody [4K5F6] (ab189377)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labelling IL-17A with ab189377 at 10 µg/ml.
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![Western blot - Anti-IL-17A antibody [4K5F6] (ab189377)](https://www.abcam.com/ps/products/189/ab189377/Images/ab189377-224029-anti-il-17a-antibody-4k5f6-western-blot.jpg)
Western blot - Anti-IL-17A antibody [4K5F6] (ab189377)All lanes : Anti-IL-17A antibody [4K5F6] (ab189377) at 0.2 µg/ml
Lane 1 : Human full-length recombinant IL-17A protein
Lane 2 : Mouse full-length recombinant IL-17A protein
Lane 3 : Rat full-length recombinant IL-17A protein
Lysates/proteins at 0.1 µg/ml per lane.
Predicted band size: 15 kDa
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![Flow Cytometry - Anti-IL-17A antibody [4K5F6] (ab189377)](https://www.abcam.com/ps/products/189/ab189377/Images/ab189377-224030-anti-il-17a-antibody-4k5f6-flow-cytometry.jpg)
Flow Cytometry - Anti-IL-17A antibody [4K5F6] (ab189377)Intracellular flow cytometric analysis of IL-17A in Human lymphocytes stimulated with PMA (5 ng/ml), Ionomycin (1 mM), and Brefeldin A (1X) for 5 hrs, labeling IL-17A with ab189377 at 0.1 ug/106 cells (red) Green represents isotype control antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.
