Biotin Anti-IL-17A antibody [3.41.2.6.1] (ab155575)
![Biotin Anti-IL-17A antibody [3.41.2.6.1] (ab155575)](https://www.abcam.com/ps/products/155/ab155575/Images/ab155575-162799-anti-il-17a-antibody-341261-biotin-sandwich-elisa.jpg "Biotin Anti-IL-17A antibody [3.41.2.6.1] (ab155575)")
Key features and details
- Biotin Mouse monoclonal [3.41.2.6.1] to IL-17A
- Suitable for: Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-IL-17A antibody [3.41.2.6.1]
See all IL-17A primary antibodies -
Description
Biotin Mouse monoclonal [3.41.2.6.1] to IL-17A -
Host species
Mouse -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species sELISA Recombinant fragment
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Immunogen
Recombinant full length protein corresponding to Human IL-17A.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 95% PBS, 4% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3.41.2.6.1 -
Isotype
IgG -
Research areas
Images
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Sandwich ELISA - Biotin Anti-IL-17A antibody [3.41.2.6.1] (ab155575)
Sandwich ELISA analysis of Human IL-17A using ab155575.
Sandwich ELISA analysis of Human IL-17A monoclonal antibody was performed by coating a blank 96-well microtiter plate with 100µl per well of Human IL-17A moncolonal antibody in duplicate at 1, 3, 4, 5, 7, and 9µg/ml in DPBS and incubating for 12-18 hours at 4C. The plate was aspirated and blocked with 300µl per well of 4% BSA and 5% sucrose in DPBS for 1 hour at room temperature. Human IL-17A recombinant protein at 50µl per well was added in duplicate at 2000, 1000, 500, 250, 125, 62.5, 31.25, and 0 pg/ml for 2 hours at room temperature along with 50µl of Human IL-17A biotinylated monoclonal antibody (ab15575) in all applicable wells at 0.1µg/ml for 2 hours at room temperature. The plate was washed with ELISA Wash Buffer and incubated with 100µl per well of Streptavidin-HRP in all test wells at 1:40,000 dilution for 30 minutes at room temperature and then washed and incubated with 100µl per well of TMB substrate for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.
