Human IL-5 ELISA Set (without plates) (ab47356)
Overview
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Product name
Human IL-5 ELISA Set (without plates) -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
7.8 pg/ml - 250 pg/mlAssay duration
Multiple steps standard assaySpecies reactivity
Reacts with: HumanProduct overview
Abcam’s IL-5 Human in vitro ELISA Set is designed for the quantitative measurement of IL-5 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA Set is intended to measure human IL-5 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
These reagents are intended for research use only, not for diagnostic purposes.
The IL-5 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human IL-5.
Tested applications
Suitable for: Sandwich ELISAmore detailsPlatform
ReagentsProperties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-IL-5 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml IL-5 Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml IL-5 standard Yellow 5 vials 10 vials 15 vials Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials -
Research areas
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Relevance
Interleukin 5 (IL-5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70% amino acid sequence homology. -
Cellular localization
Secreted -
Alternative names
- B cell differentiation factor I
- Eosinophil differentiation factor
- IL5
see all -
Database links
- Entrez Gene: 3567 Human
- SwissProt: P05113 Human
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-IL-5 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml IL-5 Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml IL-5 standard Yellow 5 vials 10 vials 15 vials Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials -
Research areas
-
Relevance
Interleukin 5 (IL-5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70% amino acid sequence homology. -
Cellular localization
Secreted -
Alternative names
- B cell differentiation factor I
- Eosinophil differentiation factor
- IL5
see all -
Database links
- Entrez Gene: 3567 Human
- SwissProt: P05113 Human
Images
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IL-5 Human ELISA Set (ab47356)IL-5 Standard curve ranging from 7.8 to 250 pg/mL