Anti-IL-1 beta antibody [EPR16805-15] (ab234437)
![Anti-IL-1 beta antibody [EPR16805-15] (ab234437)](https://www.abcam.com/ps/products/234/ab234437/Images/ab234437-3-234437IP1.jpg "Anti-IL-1 beta antibody [EPR16805-15] (ab234437)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16805-15] to IL-1 beta
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Mouse
Overview
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Product name
Anti-IL-1 beta antibody [EPR16805-15]
See all IL-1 beta primary antibodies -
Description
Rabbit monoclonal [EPR16805-15] to IL-1 beta -
Host species
Rabbit -
Specificity
IL-1 beta is not present under homeostatic conditions; it is induced and secreted only upon inflammatory signals and its secretion is tightly controlled at the levels of transcription, mRNA stability, translation, post-translational modifications and processing (PMID: 26686225).
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseIP MouseWB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: RAW 264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, then with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate. IP: RAW 264.7 treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h, whole cell lysate. Flow cyt: RAW 264.7 cells treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16805-15 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-IL-1 beta antibody [EPR16805-15] (ab234437)
IL-1 beta was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h) whole cell lysate with ab234437 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234437 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: RAW 264.7 (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h) whole cell lysate 10 μg (Input).
Lane 2: ab234437 IP in RAW 264.7 (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234437 in RAW 264.7 (treated with 100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h) whole cell lysate.IL-1 beta is not present under homeostatic conditions; it is induced and secreted only upon inflammatory signals and its secretion is tightly controlled at the levels of transcription, mRNA stability, translation, post-translational modifications and processing (PMID: 26686225).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds. -
![Flow Cytometry - Anti-IL-1 beta antibody [EPR16805-15] (ab234437)](https://www.abcam.com/ps/products/234/ab234437/Images/ab234437-2-234437FC1.jpg)
Flow Cytometry - Anti-IL-1 beta antibody [EPR16805-15] (ab234437)Flow cytometric analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells that were either treated (100 ng/ml LPS for 3h, then add 300 ng/ml Brefeldin A for 3h labeling)(red) or untreated (green) labeling IL-1 beta with ab234437 at 1/60 dilution compared with a rabbit monoclonal IgG Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti-Rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
IL-1 beta is not present under homeostatic conditions; it is induced and secreted only upon inflammatory signals and its secretion is tightly controlled at the levels of transcription, mRNA stability, translation, post-translational modifications and processing (PMID: 26686225).
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![Western blot - Anti-IL-1 beta antibody [EPR16805-15] (ab234437)](https://www.abcam.com/ps/products/234/ab234437/Images/ab234437-1-234437WB1.jpg)
Western blot - Anti-IL-1 beta antibody [EPR16805-15] (ab234437)All lanes : Anti-IL-1 beta antibody [EPR16805-15] (ab234437) at 1/1000 dilution
Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW 264.7 (treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, then with 300 ng/ml Brefeldin A (BFA) added after 3 hours) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 17,28,31 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 8446594).
IL-1 beta is not present under homeostatic conditions; it is induced and secreted only upon inflammatory signals and its secretion is tightly controlled at the levels of transcription, mRNA stability, translation, post-translational modifications and processing (PMID: 26686225).
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![Anti-IL-1 beta antibody [EPR16805-15] (ab234437)](https://www.abcam.com/ps/products/234/ab234437/Images/ab234437-4-benefits-of-recombinant-antibodies.png)
Anti-IL-1 beta antibody [EPR16805-15] (ab234437)
