Human IL-2RB ELISA Kit (CD122) (ab275903)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8 pg/ml
- Range: 43.75 pg/ml - 2800 pg/ml
- Sample type: Cell culture media, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-2RB ELISA Kit (CD122)
See all IL2 Receptor beta/p75 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4% Inter-assay Sample n Mean SD CV% Serum 3 7% -
Sample type
Serum, Cell Lysate, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8 pg/ml -
Range
43.75 pg/ml - 2800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 89 81% - 94% Serum 83 80% - 86% Cell culture media 81 79% - 84% Hep Plasma 92 84% - 97% EDTA Plasma 98 95% - 104% Cit plasma 89 87% - 90% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-2RB ELISA kit (ab275903) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-2RB protein in human serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-2RB with 8 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
IL-2RB (Interleukin-2 receptor subunit beta), also known as CD122, is a receptor for interleukin-2. IL-2 receptor exists in 3 different forms: a high affinity non-covalent dimer of an alpha and a beta subunit, an intermediate affinity monomer of beta subunit, and a low affinity monomer of alpha subunit. The high and intermediate affinity forms can also associate with a gamma subunit. The beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Desmoglein 3 (PVA) Capture Antibody 1 x 600µl 10X Human Desmoglein 3 (PVA) Detector Antibody 1 x 600ml 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Desmoglein 3 (PVA) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 1 fibronectin type-III domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD122
- CD122 antigen
- High affinity IL 2 receptor beta subunit
see all -
Database links
- Entrez Gene: 3560 Human
- Omim: 146710 Human
- SwissProt: P14784 Human
- Unigene: 474787 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human IL-2RB standard curve in Sample Diluent NS.The IL-2RB standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-2RB in human serum and plasma samples.The concentrations of IL-2RB were measured in duplicates, interpolated from the IL-2RB standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (EDTA) 100% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2RB concentration was determined to be 274 pg/mL in neat serum, 138 pg/mL in neat plasma (citrate), 155 pg/mL in neat plasma (EDTA) and 225 pg/mL in neat plasma (heparin).
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. Interpolated concentrations of native IL-2RB in human PBMC cell culture supernatant samples treated with or without 1.5% PHA-M for 36 hours.The concentrations of IL-2RB were measured in duplicates, interpolated from the IL-2RB standard curves and corrected for sample dilution. Undiluted samples are as follows: PHA treated PBMC supernatant 100% and untreated PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2RB concentration was determined to be 186 pg/mL in neat PBMC supernatant PHA-M treated and undetectable in untreated PBMC supernatant.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2RB concentration was determined to be 234 pg/mL with a range of 101 – 392 pg/mL.
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Sandwich ELISA - Human IL-2RB ELISA Kit (CD122) (ab275903)To learn more about the advantages of recombinant antibodies see here.
