Human Desmoglein 3 Antibody Pair - BSA and Azide free (PVA) (ab253456)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human Desmoglein 3 Antibody Pair - BSA and Azide free (PVA)
See all Desmoglein 3/PVA kits -
Assay type
ELISA set -
Range
39.063 pg/ml - 2500 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Both capture and detector antibodies are recombinant rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
The Antibody Pair can be used to quantify Human Desmoglein 3. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab275903), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Desmoglein 3 Capture Antibody (unconjugated) 1 x 100µg Human Desmoglein 3 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
Tissue specificity
Epidermis, tongue, tonsil, esophagus and carcinomas. -
Sequence similarities
Contains 4 cadherin domains. -
Domain
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. -
Cellular localization
Cell membrane. Cell junction > desmosome. - Information by UniProt
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Alternative names
- 130 kD pemphigus vulgaris antigen
- 130 kDa pemphigus vulgaris antigen
- Balding
see all -
Database links
- Entrez Gene: 1830 Human
- Omim: 169615 Human
- SwissProt: P32926 Human
- Unigene: 1925 Human
Images
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Sandwich ELISA - Human Desmoglein 3 Antibody Pair - BSA and Azide free (PVA) (ab253456)To learn more about the advantages of recombinant antibodies see here.
