Anti-IKB epsilon antibody (ab230901)
Key features and details
- Rabbit polyclonal to IKB epsilon
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IKB epsilon antibody
See all IKB epsilon primary antibodies -
Description
Rabbit polyclonal to IKB epsilon -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow
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Immunogen
Synthetic peptide corresponding to Human IKB epsilon (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: O00221 -
Positive control
- WB: Jurkat, HeLa and PC-12 whole cell lysates. IHC-P: Human heart tissue. ICC/IF: HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IKB epsilon antibody (ab230901)All lanes : Anti-IKB epsilon antibody (ab230901) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 53 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB epsilon antibody (ab230901)Formalin-fixed, paraffin-embedded human heart tissue stained for IKB epsilon with ab230901 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-IKB epsilon antibody (ab230901)Formalin-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for IKB epsilon (red) using ab230901 at 1/100 dilution followed by DyLight 594-conjugated secondary antibody in ICC/IF.
The nuclear counter stain is DAPI (blue).
