Anti-IKB epsilon (phospho S22) antibody (ab75907)
Key features and details
- Rabbit polyclonal to IKB epsilon (phospho S22)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IKB epsilon (phospho S22) antibody
See all IKB epsilon primary antibodies -
Description
Rabbit polyclonal to IKB epsilon (phospho S22) -
Host species
Rabbit -
Specificity
ab75907 detects endogenous levels of IKB epsilon only when phosphorylated at serine 22. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from human IKB epsilon around the phosphorylation site of serine 22 (I-E-SP-L-R).
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Positive control
- Human breast carcinoma tissue; 293 cell extracts treated with TNF-alpha (20ng/ml, 15min).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab75907 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB epsilon (phospho S22) antibody (ab75907)ab75907 at 1/50 dilution staining IKB epsilon in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising peptide.
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Western blot - Anti-IKB epsilon (phospho S22) antibody (ab75907)All lanes : Anti-IKB epsilon (phospho S22) antibody (ab75907) at 1/500 dilution
Lane 1 : 293 cell extracts, untreated
Lane 2 : 293 cell extracts, treated with TNF-alpha (20ng/ml, 15min)
Predicted band size: 38 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
