Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸ 10 µl 134 016 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP4420] to IgG4
Suitable for: WB, IHC-P
Reacts with: Human, Recombinant fragment

Product name

Anti-IgG4 antibody [EP4420]
See all IgG4 primary antibodies
Description

Rabbit monoclonal [EP4420] to IgG4
Host species

Rabbit
Specificity

Does not cross-react with IgG1, IgG2, or IgG3.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Recombinant fragment

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human IgG4 recombinant protein; Human plasma; Human spleen tissue lysate. IHC-P: Human tonsil tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP4420
Isotype

IgG
Research areas

Western blot - Anti-IgG4 antibody [EP4420] (ab109493)

All lanes : Anti-IgG4 antibody [EP4420] (ab109493) at 1/1000 dilution

Lane 1 : IgG4 Recombinant protein at 0.005 µg
Lane 2 : IgG1 Recombinant protein at 0.01 µg
Lane 3 : IgG2 Recombinant protein at 0.01 µg
Lane 4 : IgG3 Recombinant protein at 0.01 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 52 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 180 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [EP4420] (ab109493)

ab109493 at 1/1000 dilution staining IgG4 in Paraffin embedded Human tonsil tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Western blot - Anti-IgG4 antibody [EP4420] (ab109493)

All lanes : Anti-IgG4 antibody [EP4420] (ab109493) at 1/1000 dilution

Lane 1 : IgG4 Recombinant protein at 0.005 µg
Lane 2 : IgG1 Recombinant protein at 0.01 µg
Lane 3 : IgG2 Recombinant protein at 0.01 µg
Lane 4 : IgG3 Recombinant protein at 0.01 µg
Lane 5 : Human spleen lysate at 10 µg
Lane 6 : Human plasma lysate at 10 µg

Predicted band size: 36 kDa
Anti-IgG4 antibody [EP4420] (ab109493)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com