Anti-IgG4 antibody (ab232869)
Key features and details
- Rabbit polyclonal to IgG4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-IgG4 antibody
See all IgG4 primary antibodies -
Description
Rabbit polyclonal to IgG4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Pig -
Immunogen
Recombinant fragment corresponding to Human IgG4 aa 200 to the C-terminus. Expressed in E.coli.
Database link: P01861 -
Positive control
- WB: Recombinant human IgG4; human, pig and mouse serum. IHC-P: Human prostate cancer, glioma, kidney and stomach tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody (ab232869)
Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for IgG4 using ab232869 at 20μg/ml in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody (ab232869)Formalin-fixed, paraffin-embedded human glioma tissue stained for IgG4 using ab232869 at 20μg/ml in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody (ab232869)Formalin-fixed, paraffin-embedded human kidney tissue stained for IgG4 using ab232869 at 20μg/ml in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody (ab232869)Formalin-fixed, paraffin-embedded human stomach tissue stained for IgG4 using ab232869 at 20μg/ml in immunohistochemical analysis.
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Western blot - Anti-IgG4 antibody (ab232869)Anti-IgG4 antibody (ab232869) at 3 µg/ml + Recombinant human IgG4
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
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Western blot - Anti-IgG4 antibody (ab232869)All lanes : Anti-IgG4 antibody (ab232869) at 3 µg/ml
Lane 1 : Human serum
Lane 2 : Pig serum
Lane 3 : Mouse serum
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
