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Immunology Immunoglobulins Heavy Chain IgG

Anti-IgG antibody [RIGG-69] - BSA and Azide free (ab248512)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-IgG antibody [RIGG-69] - BSA and Azide free (ab248512)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RIGG-69] to IgG - BSA and Azide free
  • Suitable for: WB, ELISA
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-IgG antibody [RIGG-69] - BSA and Azide free
    See all IgG primary antibodies
  • Description

    Rabbit monoclonal [RIGG-69] to IgG - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length protein corresponding to Rat IgG.

  • General notes

    Ab248512 is the carrier-free version of ab133470. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248512 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    RIGG-69
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG

Images

  • Western blot - Anti-IgG antibody [RIGG-69] - BSA and Azide free (ab248512)
    Western blot - Anti-IgG antibody [RIGG-69] - BSA and Azide free (ab248512)
    All lanes : purified at 1/1000 dilution

    Lane 1 : Human tonsil tissue lysate
    Lane 2 : Human heart tissue lysate
    Lane 3 : Mouse heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab133470, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • ELISA - Anti-IgG antibody [RIGG-69] - BSA and Azide free (ab248512)
    ELISA - Anti-IgG antibody [RIGG-69] - BSA and Azide free (ab248512)
    This data was developed using ab133470, the same antibody clone in a different buffer formulation.Direct ELISA antibody dose-response curve using ab133470 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
  • Western blot - Anti-IgG antibody [RIGG-69] - BSA and Azide free (ab248512)
    Western blot - Anti-IgG antibody [RIGG-69] - BSA and Azide free (ab248512)
    All lanes : Anti-IgG antibody [RIGG-69] (ab133470) at 1/150 dilution (unpurified)

    Lane 1 : Human tonsil tissue lysate
    Lane 2 : Human heart tissue lysate
    Lane 3 : Mouse heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Observed band size: 50 kDa why is the actual band size different from the predicted?



    This data was developed using ab133470, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • ELISA - Anti-IgG antibody [RIGG-69] - BSA and Azide free (ab248512)
    ELISA - Anti-IgG antibody [RIGG-69] - BSA and Azide free (ab248512)
    This data was developed using ab133470, the same antibody clone in a different buffer formulation.Antibody dose-response curve using purified ab133470. Antigen concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
  • Western blot - Anti-IgG antibody [RIGG-69] - BSA and Azide free (ab248512)
    Western blot - Anti-IgG antibody [RIGG-69] - BSA and Azide free (ab248512)
    All lanes : Anti-IgG antibody [RIGG-69] (ab133470) (unpurified)

    Lane 1 : Rat spleen tissue lysate
    Lane 2 : Human tonsil tissue lysate


    This data was developed using ab133470, the same antibody clone in a different buffer formulation.

  • ELISA - Anti-IgG antibody [RIGG-69] - BSA and Azide free (ab248512)
    ELISA - Anti-IgG antibody [RIGG-69] - BSA and Azide free (ab248512)
    This data was developed using ab133470, the same antibody clone in a different buffer formulation.Antibody dose-response curve using unpurified ab133470. Antigen concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
  • Anti-IgG antibody [RIGG-69] - BSA and Azide free (ab248512)
    Anti-IgG antibody [RIGG-69] - BSA and Azide free (ab248512)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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