Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IDH1 antibody [RcMab-1] (ab256557)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [RcMab-1] to IDH1
  • Suitable for: IHC-P, ICC/IF, WB
  • Knockout validated
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-IDH1 antibody [RcMab-1]
    See all IDH1 primary antibodies

  • Description

    Rat monoclonal [RcMab-1] to IDH1

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human IDH1.
    Database link: O75874

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human prostatic hyperplasia tissue and kidney tissue. WB: Wild type HAP1 whole cell lysate. HeLa, PC-12, HepG2 and Raji whole cell lysate. Human and rat heart tissue lysate. ICC/IF: Wild type HAP1 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-IDH1 antibody [RcMab-1] (ab256557)
    Western blot - Anti-IDH1 antibody [RcMab-1] (ab256557)
    All lanes : Anti-IDH1 antibody [RcMab-1] (ab256557) at 1.479 µg/ml

    Lane 1 : Wild type HAP1 whole cell lysate
    Lane 2 : IDH1 knockout HAP1 whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 5 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 46 kDa


    Exposure time: 8 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-IDH1 antibody [RcMab-1] (ab256557)
    Western blot - Anti-IDH1 antibody [RcMab-1] (ab256557)
    All lanes : Anti-IDH1 antibody [RcMab-1] (ab256557) at 1.479 µg/ml

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
    Lane 2 : Rat heart tissue lysate
    Lane 3 : Human heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 46 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lanes 2-3: 3 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] (ab256557)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] (ab256557)

    Immunohistochemical analysis of paraffin-embedded human prostatic hyperplasia tissue labeling IDH1 with ab256557 at 1/500 dilution (14.79µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on human prostatic hyperplasia. The section was incubated with ab256557 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] (ab256557)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] (ab256557)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling IDH1 with ab256557 at 1/500 dilution (14.79µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on human kidney tissue. The section was incubated with ab256557 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] (ab256557)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [RcMab-1] (ab256557)

    Immunohistochemical analysis of paraffin-embedded human normal cerebrum tissue labeling IDH1 with ab256557 at 1/500 dilution (14.79µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). The section was incubated with ab256557 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Negative control: No staining on human normal cerebrum.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-IDH1 antibody [RcMab-1] (ab256557)
    Immunocytochemistry/ Immunofluorescence - Anti-IDH1 antibody [RcMab-1] (ab256557)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized wild-type HAP1 cells labelling IDH1 with ab256557 at 1/20 dilution (73.95µg/ml), followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

    Confocal image showing positive staining in wild-type HAP1 cells, while no staining in IDH1 knockout HAP1 cells.

    ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only controls.

  • Anti-IDH1 antibody [RcMab-1] (ab256557)
    Anti-IDH1 antibody [RcMab-1] (ab256557)

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