Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free (ab251070)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15931] to NDUFAF4 - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free
    See all NDUFAF4 primary antibodies

  • Description

    Rabbit monoclonal [EPR15931] to NDUFAF4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251070 is the carrier-free version of ab191414. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251070 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Images

  • Immunocytochemistry - Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free (ab251070)
    Immunocytochemistry - Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free (ab251070)
    This data was developed using ab191414, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells labeling NDUFAF4 using ab191414 at 1/150 dilution (9.5 μg/mL). A Goat anti Rabbit IgG (Alexa Fluor488) at 1/200 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% Paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI. The 2 negative cotrols images were obtained using Goat anti Mouse IgG (Alexa Fluor®594) at 1:400 dilution as secondary antibody.
  • Western blot - Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free (ab251070)
    Western blot - Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free (ab251070)
    All lanes : Anti-NDUFAF4 antibody [EPR15931] (ab191414) at 1/10000 dilution

    Lane 1 : T47-D cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : MDA-MB-435 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa



    This data was developed using ab191414, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free (ab251070)
    Western blot - Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free (ab251070)
    Anti-NDUFAF4 antibody [EPR15931] (ab191414) at 1/1000 dilution + MCF7 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa



    This data was developed using ab191414, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free (ab251070)
    Immunoprecipitation - Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free (ab251070)
    This data was developed using ab191414, the same antibody clone in a different buffer formulation.Immunoprecipitation analysis of MDA-MB-435 cell lysate labeling NDUFAF4 using ab191414 at 1/90 dilution (Lane 1). An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of MDA-MB-435 cell lysate.
  • Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free (ab251070)
    Anti-NDUFAF4 antibody [EPR15931] - BSA and Azide free (ab251070)

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