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Epigenetics and Nuclear Signaling Chromosome Structure Scaffold Proteins

Anti-IB-1 antibody (ab230341)

Anti-IB-1 antibody (ab230341)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to IB-1
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-IB-1 antibody
    See all IB-1 primary antibodies
  • Description

    Rabbit polyclonal to IB-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human IB-1 aa 450-550.
    Database link: Q9UQF2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: MCF7 cell lysate. IHC-P: Human kidney tissue. ICC/IF: MCF7 cells. Flow Cyt (Intra): MCF7 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Scaffold Proteins

Images

  • Western blot - Anti-IB-1 antibody (ab230341)
    Western blot - Anti-IB-1 antibody (ab230341)
    Anti-IB-1 antibody (ab230341) at 1/1000 dilution + MCF7 (human breast adenocarcinoma cell line) cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 78 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IB-1 antibody (ab230341)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IB-1 antibody (ab230341)

    Formalin-fixed, paraffin-embedded human kidney tissue stained for IB-1 with ab230341 at 1/100 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-IB-1 antibody (ab230341)
    Immunocytochemistry/ Immunofluorescence - Anti-IB-1 antibody (ab230341)

    MCF7 (human breast adenocarcinoma cell line) cells stained for IB-1 (green) using ab230341 at 1/10 dilution followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG in ICC/IF.  

    The nuclear counter stain is DAPI (blue).

  • Flow Cytometry - Anti-IB-1 antibody (ab230341)
    Flow Cytometry - Anti-IB-1 antibody (ab230341)

    Flow cytometric analysis of MCF7 (human breast adenocarcinoma cell line) cells  labeling IB-1 with ab230341 at 1/10 dilution (green) compared with a negative control control (blue).

    FITC-conjugated goat-anti-rabbit secondary antibodies were used.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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