Anti-IB-1 antibody (ab24449)
Key features and details
- Rabbit polyclonal to IB-1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IB-1 antibody
See all IB-1 primary antibodies -
Description
Rabbit polyclonal to IB-1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse IB-1 (N terminal).
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Positive control
- Mouse Intestine. Rat kidney. Jurkat whole cell lysate (ab7899).
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 3664 JIP-1 Antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IB-1 antibody (ab24449)All lanes : Anti-IB-1 antibody (ab24449)
Lane 1 : Rat kidney
Lane 2 : Mouse Intestine
Lane 3 : Jurkat Cell lysate
Predicted band size: 78 kDa
Observed band size: 78 kDa
Additional bands at: 110 kDa (possible isoform)
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Immunocytochemistry/ Immunofluorescence - Anti-IB-1 antibody (ab24449)ICC/IF image of ab24449 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24449, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
