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Neuroscience Neurotransmitter Biogenic Amines Noradrenaline

Anti-PNMT antibody (ab90862)

Price and availability

247 929 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-PNMT antibody (ab90862)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PNMT
  • Suitable for: WB
  • Reacts with: Rat
  • Isotype: IgG

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Overview

  • Product name

    Anti-PNMT antibody
    See all PNMT primary antibodies
  • Description

    Rabbit polyclonal to PNMT
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human PNMT aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab91000)

  • Positive control

    • This antibody gave a positive signal in Rat Adrenal gland Tissue Lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Noradrenaline
    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Adrenaline
    • Cardiovascular
    • Vasculature
    • Vasodilation
    • Catecholamines
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human PNMT protein (ab89368)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab90862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Rat
All applications
Mouse
Human
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).

Target

  • Function

    Converts noradrenaline to adrenaline.
  • Pathway

    Catecholamine biosynthesis; (R)-adrenaline biosynthesis; (R)-adrenaline from (R)-noradrenaline: step 1/1.
  • Sequence similarities

    Belongs to the NNMT/PNMT/TEMT family.
  • Target information above from: UniProt accession P11086 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5409 Human
    • Entrez Gene: 18948 Mouse
    • Entrez Gene: 24661 Rat
    • Omim: 171190 Human
    • SwissProt: P11086 Human
    • SwissProt: P40935 Mouse
    • SwissProt: P10937 Rat
    • Unigene: 1892 Human
    • Unigene: 57030 Mouse
    • Unigene: 202604 Rat
    see all
  • Alternative names

    • Noradrenaline N-methyltransferase antibody
    • PENT antibody
    • Phenylethanolamine N-methyltransferase antibody
    • PNMT antibody
    • PNMT_HUMAN antibody
    • PNMTase antibody
    see all

Images

  • Western blot - Anti-PNMT antibody (ab90862)
    Western blot - Anti-PNMT antibody (ab90862)
    Anti-PNMT antibody (ab90862) at 1 µg/ml + Adrenal gland (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 31 kDa
    Observed band size: 31 kDa


    Exposure time: 10 minutes

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab90862? Please let us know so that we can cite the reference in this datasheet.

    ab90862 has been referenced in 1 publication.

    • Bechmann N  et al. Impact of Extrinsic and Intrinsic Hypoxia on Catecholamine Biosynthesis in Absence or Presence of Hif2a in Pheochromocytoma Cells. Cancers (Basel) 11:N/A (2019). PubMed: 31035382

    Images

    • Western blot - Anti-PNMT antibody (ab90862)
      Western blot - Anti-PNMT antibody (ab90862)
      Anti-PNMT antibody (ab90862) at 1 µg/ml + Adrenal gland (Rat) Tissue Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 31 kDa
      Observed band size: 31 kDa


      Exposure time: 10 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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      Applications: IHC-P, WB

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