Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671)
![Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671)](https://www.abcam.com/ps/products/140/ab140671/Images/ab140671-156089-anti-hyaluronan-synthase-2-antibody-4e7-immunohistochemistry.jpg "Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671)")
Key features and details
- Mouse monoclonal [4E7] to Hyaluronan synthase 2
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Hyaluronan synthase 2 antibody [4E7]
See all Hyaluronan synthase 2 primary antibodies -
Description
Mouse monoclonal [4E7] to Hyaluronan synthase 2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Purified recombinant fragment, corresponding to amino acids 67-170 of Human Hyaluronan synthase 2, expressed in E. coli.
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Positive control
- Recombinant Human Hyaluronan synthase 2 protein; NTERA2 and HEK293 cell lysates; HeLa cells. Human ovarian cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer. -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4E7 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671)Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Hyaluronan synthase 2 with ab140671 at 1/200 dilution followed by DAB staining.
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![Immunocytochemistry/ Immunofluorescence - Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671)](https://www.abcam.com/ps/products/140/ab140671/Images/ab140671-156090-anti-hyaluronan-synthase-2-antibody-4e7-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671)Immunofluorescenct analysis of HeLa cells labelling Hyaluronan synthase 2 with ab140671 at 1/100 dilution (green); Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. -
![Western blot - Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671)](https://www.abcam.com/ps/products/140/ab140671/Images/ab140671-156088-anti-hyaluronan-synthase-2-antibody-4e7-western-blot.jpg)
Western blot - Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671)Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671) at 1/500 dilution + Recombinant Human Hyaluronan synthase 2 protein
Predicted band size: 64 kDa
Expected MW is 37.5 kDa -
![Western blot - Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671)](https://www.abcam.com/ps/products/140/ab140671/Images/ab140671-156087-anti-hyaluronan-synthase-2-antibody-4e7-western-blot.jpg)
Western blot - Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671)All lanes : Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671) at 1/500 dilution
Lane 1 : NTERA2 cell lysate
Lane 2 : HEK293 cell lysate
Predicted band size: 64 kDa
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![Flow Cytometry - Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671)](https://www.abcam.com/ps/products/140/ab140671/Images/ab140671-204438-anti-hyaluronan-synthase-2-antibody-4e7-flow-cytometry.jpg)
Flow Cytometry - Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671)Overlay histogram showing HeLa cells stained with ab140671 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140671, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
