Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Hyaluronan synthase 2 with ab140671 at 1/200 dilution followed by DAB staining.

Immunofluorescenct analysis of HeLa cells labelling Hyaluronan synthase 2 with ab140671 at 1/100 dilution (green); Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671) at 1/500 dilution + Recombinant Human Hyaluronan synthase 2 protein

Predicted band size: 64 kDa



Expected MW is 37.5 kDa

All lanes : Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671) at 1/500 dilution

Lane 1 : NTERA2 cell lysate
Lane 2 : HEK293 cell lysate

Predicted band size: 64 kDa

Overlay histogram showing HeLa cells stained with ab140671 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140671, 0.1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.