Anti-Hyaluronan synthase 2 antibody (ab131364)
Key features and details
- Rabbit polyclonal to Hyaluronan synthase 2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Hyaluronan synthase 2 antibody
See all Hyaluronan synthase 2 primary antibodies -
Description
Rabbit polyclonal to Hyaluronan synthase 2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
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Immunogen
This product was produced with the following immunogens:
This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide aa 450-550.
Database link: Q92819 -
Positive control
- This antibody gave a positive signal in Human Heart tissue lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab131364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa). Abcam recommends using milk as the blocking agent (3%). Target
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Function
Plays a role in hyaluronan/hyaluronic acid (HA) synthesis. -
Tissue specificity
Expressed in fibroblasts. -
Pathway
Glycan biosynthesis; hyaluronan biosynthesis. -
Involvement in disease
Note=A chromosomal aberration involving HAS2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. 8q12.1 to 8q24.1 intrachromosomal rearrangement with PLAG1. -
Sequence similarities
Belongs to the nodC/HAS family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 281220 Cow
- Entrez Gene: 3037 Human
- Entrez Gene: 15117 Mouse
- Entrez Gene: 25694 Rat
- Omim: 601636 Human
- SwissProt: O97711 Cow
- SwissProt: Q92819 Human
- SwissProt: P70312 Mouse
see all -
Alternative names
- HA synthase 2 antibody
- has2 antibody
- HAS2_HUMAN antibody
see all
Images
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Western blot - Anti-Hyaluronan synthase 2 antibody (ab131364)Anti-Hyaluronan synthase 2 antibody (ab131364) at 1 µg/ml + Human heart tissue lysate at 25 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 50000 cells
Predicted band size: 63 kDa
Observed band size: 60,77 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesBlocking buffer: 3% Milk
Gel type: MOPS
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Western blot - Anti-Hyaluronan synthase 2 antibody (ab131364)Anti-Hyaluronan synthase 2 antibody (ab131364) at 1 µg/ml (3% milk blocking) + Human heart tissue lysate - total protein (ab29431) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 135 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Abcam recommends using milk as the blocking agent - 3%. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab131364 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (3)
ab131364 has been referenced in 3 publications.
- Pan Y et al. Estrogen improves the development of yak (Bos grunniens) oocytes by targeting cumulus expansion and levels of oocyte-secreted factors during in vitro maturation. PLoS One 15:e0239151 (2020). PubMed: 32941516
- Yang H et al. Berberine inhibits low shear stress-induced glycocalyx degradation via modulating AMPK and p47phox/Hyal2 signal pathway. Eur J Pharmacol 856:172413 (2019). PubMed: 31152700
- Zhang Y et al. MicroRNA-410 promotes chondrogenic differentiation of human bone marrow mesenchymal stem cells through down-regulating Wnt3a. Am J Transl Res 9:136-145 (2017). WB ; Human . PubMed: 28123640
Images
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Western blot - Anti-Hyaluronan synthase 2 antibody (ab131364)Anti-Hyaluronan synthase 2 antibody (ab131364) at 1 µg/ml + Human heart tissue lysate at 25 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 50000 cells
Predicted band size: 63 kDa
Observed band size: 60,77 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesBlocking buffer: 3% Milk
Gel type: MOPS
-
Western blot - Anti-Hyaluronan synthase 2 antibody (ab131364)Anti-Hyaluronan synthase 2 antibody (ab131364) at 1 µg/ml (3% milk blocking) + Human heart tissue lysate - total protein (ab29431) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 135 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Abcam recommends using milk as the blocking agent - 3%. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab131364 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
