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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin

Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)

Price and availability

281 433 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP9447] to HSPC150/UBE2T
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-HSPC150/UBE2T antibody [EP9447]
    See all HSPC150/UBE2T primary antibodies
  • Description

    Rabbit monoclonal [EP9447] to HSPC150/UBE2T
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    within Human HSPC150/UBE2T aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NPD8

  • Positive control

    • HeLa, Jurkat and 293T cell lysates; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as HSPC150

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP9447
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • Other E3 Ligases
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • E2 Ubiquitin Conjugating Enzymes

Images

  • Western blot - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)
    Western blot - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)
    All lanes : Anti-HSPC150/UBE2T antibody [EP9447] (ab179802) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 23 kDa

  • Immunoprecipitation - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)
    Immunoprecipitation - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)

    Western blot analysis on immunoprecipitation pellet from (1) Jurkat cell lysate or (2) 1X PBS (negative control) using ab179802, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Immunocytochemistry/ Immunofluorescence - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)
    Immunocytochemistry/ Immunofluorescence - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)

    Immunofluorescent analysis of HeLa cells labeling HSPC150/UBE2T with ab179802 at 1/50 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)

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    Applications: WB

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    Anti-HSPC150/UBE2T antibody (ab154022)

    Applications: IHC-P, WB

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