Recombinant human Ubiquitin 7-amido-4-methylcoumarin protein (ab206162)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Ubiquitin 7-amido-4-methylcoumarin protein -
Biological activity
The concentration of ab206162 used in deubiquitinating reactions is usually 0.1-1 µM. The released AMC fluorescence can be monitored using a fluorometer or plate reader with excitation and emission wavelengths at 360 nm and 460 nm, respectively.
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Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL EDGRTLSDYN IQKESTLHLVLRLRGG -
Predicted molecular weight
9 kDa -
Amino acids
1 to 76
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Preparation and Storage
- Ub-AMC
- Ubiquitin-AMC
Images
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Functional Studies - Recombinant human Ubiquitin 7-amido-4-methylcoumarin protein (ab206162)
Functional analysis of ab206162: The reaction contained 2 nM bovine 26S proteasome and 1 mM ab206162. AMC fluorescence was monitored by a plate reader using excitation and emission filter set at 360/40 and 460/30 nm, respectively.
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SDS-PAGE - Recombinant human Ubiquitin 7-amido-4-methylcoumarin protein (ab206162)SDS-PAGE analysis of ab206162, Coomassie-stained (5 µg).