Anti-RAP1GAP antibody [Y135] (ab32119)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y135] to RAP1GAP
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-RAP1GAP antibody [Y135]
See all RAP1GAP primary antibodies -
Description
Rabbit monoclonal [Y135] to RAP1GAP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human RAP1GAP aa 600-700 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: Jurkat cell lysate. IHC-P: Human stomach carcinoma.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
Y135 -
Isotype
IgG -
Research areas
Images
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Anti-RAP1GAP antibody [Y135] (ab32119) at 1/10000 dilution + Jurkat cell lysate
Predicted band size: 73 kDa
Observed band size: 85-95 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
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Ab32119, at a dilution of 1/100, staining RAP1GA1 in paraffin embedded human stomach carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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