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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin

Anti-UFD1L antibody [EPR12847] - BSA and Azide free (ab250330)

Anti-UFD1L antibody [EPR12847] - BSA and Azide free (ab250330)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12847] to UFD1L - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-UFD1L antibody [EPR12847] - BSA and Azide free
    See all UFD1L primary antibodies
  • Description

    Rabbit monoclonal [EPR12847] to UFD1L - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250330 is the carrier-free version of ab181080. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250330 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12847
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases

Images

  • Western blot - Anti-UFD1L antibody [EPR12847] - BSA and Azide free (ab250330)
    Western blot - Anti-UFD1L antibody [EPR12847] - BSA and Azide free (ab250330)
    All lanes : Anti-UFD1L antibody [EPR12847] - N-terminal (ab181080) at 1/2000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 1/1000 dilution per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa



    This data was developed using ab181080, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-UFD1L antibody [EPR12847] - BSA and Azide free (ab250330)
    Immunocytochemistry - Anti-UFD1L antibody [EPR12847] - BSA and Azide free (ab250330)
    This data was developed using ab181080, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed A431 cells labeling UFD1L with ab181080 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Dylight 488) at 1/200 dilution. Conterstained with Dapi (blue).
  • Flow Cytometry - Anti-UFD1L antibody [EPR12847] - BSA and Azide free (ab250330)
    Flow Cytometry - Anti-UFD1L antibody [EPR12847] - BSA and Azide free (ab250330)
    This data was developed using ab181080, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 293 cells (2% paraformaldehyde-fixed) labeling UFD1L with ab181080 at 1/30 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control: Rabbit IgG
  • Anti-UFD1L antibody [EPR12847] - BSA and Azide free (ab250330)
    Anti-UFD1L antibody [EPR12847] - BSA and Azide free (ab250330)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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