Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hsp70 antibody [C7] (ab239732)

Key features and details

  • Mouse monoclonal [C7] to Hsp70
  • Suitable for: WB, IHC-P
  • Reacts with: Human, Pig
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Hsp70 antibody [C7]
    See all Hsp70 primary antibodies

  • Description

    Mouse monoclonal [C7] to Hsp70

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    Pig
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human Hsp70 aa 250 to the C-terminus. N-terminal His-tag and T7-tag (Expressed in E.coli).
    Database link: P0DMV8

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Recombinant human Hsp70 protein; Pig cerebrum and liver lysate; HeLa and Jurkat cell lysate. IHC-P: Human breast cancer and prostate tissues.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS.

  • Clonality

    Monoclonal

  • Clone number

    C7

  • Isotype

    IgG2b

  • Light chain type

    kappa

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [C7] (ab239732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [C7] (ab239732)

    Paraffin-embedded human breast cancer tissue stained for Hsp70 using ab239732 at 20 μg/ml in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-Hsp70 antibody [C7] (ab239732)
    Western blot - Anti-Hsp70 antibody [C7] (ab239732)
    Anti-Hsp70 antibody [C7] (ab239732) at 3 µg/ml + Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

    Secondary
    HRP-Linked Goat anti-Mouse IgG at 0.2 µg/ml
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [C7] (ab239732)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [C7] (ab239732)

    Paraffin-embedded human prostate tissue stained for Hsp70 using ab239732 at 20 μg/ml in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-Hsp70 antibody [C7] (ab239732)
    Western blot - Anti-Hsp70 antibody [C7] (ab239732)
    Anti-Hsp70 antibody [C7] (ab239732) at 3 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Secondary
    HRP-Linked Goat anti-Mouse IgG at 0.2 µg/ml
  • Western blot - Anti-Hsp70 antibody [C7] (ab239732)
    Western blot - Anti-Hsp70 antibody [C7] (ab239732)
    Anti-Hsp70 antibody [C7] (ab239732) at 3 µg/ml + Pig liver lysate

    Secondary
    HRP-Linked Goat anti-Mouse IgG at 0.2 µg/ml
  • Western blot - Anti-Hsp70 antibody [C7] (ab239732)
    Western blot - Anti-Hsp70 antibody [C7] (ab239732)
    Anti-Hsp70 antibody [C7] (ab239732) at 3 µg/ml + Pig cerebrum lysate

    Secondary
    HRP-Linked Goat anti-Mouse IgG at 0.2 µg/ml
  • Western blot - Anti-Hsp70 antibody [C7] (ab239732)
    Western blot - Anti-Hsp70 antibody [C7] (ab239732)
    Anti-Hsp70 antibody [C7] (ab239732) at 5 µg/ml + Recombinant human Hsp70 protein

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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