Anti-Hsp70 antibody (ab31010)
Key features and details
- Rabbit polyclonal to Hsp70
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Hsp70 antibody
See all Hsp70 primary antibodies -
Description
Rabbit polyclonal to Hsp70 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
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Immunogen
Synthetic peptide within Human Hsp70 aa 550 to the C-terminus. The exact sequence is proprietary.
Database link: P0DMV8 -
Positive control
- Hela cells. Breast cancer.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab31010 is an affinity purified IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Hsp70 antibody (ab31010)
Hsp70 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Hsp70 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31010.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 70kDa; Hsp70
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody (ab31010)Tissue: Human breast cancer (formalin fixed/paraffin).
Staining: ab31010 at 1/100 dilution for 30 minutes at room temperature. -
Western blot - Anti-Hsp70 antibody (ab31010)Anti-Hsp70 antibody (ab31010) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 70 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Additional bands at: 76 kDa (possible post-translational modification)
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Western blot - Anti-Hsp70 antibody (ab31010)Anti-Hsp70 antibody (ab31010) at 1/25 dilution + 293 cell lysate
Predicted band size: 70 kDa
Observed band size: 70 kDa
