Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)
![Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)](https://www.abcam.com/ps/products/247/ab247352/Images/ab247352-449183-anti-hsp27-antibody-ep1724y-western-blot-hela-lysates.jpg "Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1724Y] to Hsp27 - BSA and Azide free
- Suitable for: ICC, WB, IHC-P
- Knockout validated
- Reacts with: Human
Overview
-
Product name
Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free
See all Hsp27 primary antibodies -
Description
Rabbit monoclonal [EP1724Y] to Hsp27 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HAP1, HeLa and MCF7 cell lysates.
-
General notes
ab247352 is the carrier-free version of ab62339 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247352 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP1724Y -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)All lanes : Anti-Hsp27 antibody [EP1724Y] (ab62339) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : HSPB1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab62339).
Lanes 1- 2: Merged signal (red and green). Green - ab62339 observed at 23 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab62339 was shown to react with Hsp27 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261738 (knockout cell lysate ab256945) was used. Wild-type HeLa and HSPB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab62339 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 500 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)](https://www.abcam.com/ps/products/247/ab247352/Images/ab247352-449530-anti-hsp27-antibody-ep1724y-western-blot-hap1-hela-mcf7.jpg)
Western blot - Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)All lanes : Anti-Hsp27 antibody [EP1724Y] (ab62339) at 1/500 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : Hsp27 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 23 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab62339).
Lanes 1 - 4: Merged signal (red and green). Green - ab62339 observed at 27 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab62339 was shown to specifically react with Hsp27 in wild-type HAP1 cells. No band was observed when Hsp27 knockout samples were used. Wild-type and Hsp27 knockout samples were subjected to SDS-PAGE. Ab62339 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)](https://www.abcam.com/ps/products/247/ab247352/Images/ab247352-2-benefits-of-recombinant-antibodies.png)
Anti-Hsp27 antibody [EP1724Y] - BSA and Azide free (ab247352)
