Anti-hSET1/SET1 antibody [BLR024E] (ab243881)
![Anti-hSET1/SET1 antibody [BLR024E] (ab243881)](https://www.abcam.com/ps/products/243/ab243881/Images/ab243881-334316-anti-hset1-antibody-blr024e-immunohistochemistry.jpg "Anti-hSET1/SET1 antibody [BLR024E] (ab243881)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR024E] to hSET1/SET1
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-hSET1/SET1 antibody [BLR024E]
See all hSET1/SET1 primary antibodies -
Description
Rabbit monoclonal [BLR024E] to hSET1/SET1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human hSET1/SET1 aa 1200-1250. The exact sequence is proprietary. Gene ID 9739.
Database link: O15047 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
This product was previously labelled as hSET1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR024E -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSET1/SET1 antibody [BLR024E] (ab243881)
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for hSET1/SET1 using ab243881 at 1/25 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSET1/SET1 antibody [BLR024E] (ab243881)](https://www.abcam.com/ps/products/243/ab243881/Images/ab243881-334315-anti-hset1-antibody-blr024e-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSET1/SET1 antibody [BLR024E] (ab243881)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for hSET1/SET1 using ab243881 at 1/25 dilution in immunohistochemical analysis. A DyLight594-conjugated goat anti-rabbit antibody was used as the secondary. The nuclear counter stain is DAPI (blue)
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![Immunoprecipitation - Anti-hSET1/SET1 antibody [BLR024E] (ab243881)](https://www.abcam.com/ps/products/243/ab243881/Images/ab243881-334314-anti-hset1-antibody-blr024e-immunoprecipitation.jpg)
Immunoprecipitation - Anti-hSET1/SET1 antibody [BLR024E] (ab243881)hSET1/SET1 was immunoprecipitated from 1.0 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab243881 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243881 at 1/1000 dilution.
Lane 1: ab243881 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
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![Western blot - Anti-hSET1/SET1 antibody [BLR024E] (ab243881)](https://www.abcam.com/ps/products/243/ab243881/Images/ab243881-334313-anti-hset1-antibody-blr024e-western-blot.jpg)
Western blot - Anti-hSET1/SET1 antibody [BLR024E] (ab243881)Western blot analysis using ab243881 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate.
Lane 2: HEK-293T whole cell lysate .
Lane3: Jurkat whole cell lysate.
Lane 4: TCMK-1 whole cell lysate.
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary.
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![Anti-hSET1/SET1 antibody [BLR024E] (ab243881)](https://www.abcam.com/ps/products/243/ab243881/Images/ab243881-5-benefits-of-recombinant-antibodies.png)
Anti-hSET1/SET1 antibody [BLR024E] (ab243881)
