Antibodies, Proteins, Kits and Reagents for Life Science

Anti-hSET1/SET1 antibody [BLR024E] (ab243881)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR024E] to hSET1/SET1
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Human

Product name

Anti-hSET1/SET1 antibody [BLR024E]
See all hSET1/SET1 primary antibodies
Description

Rabbit monoclonal [BLR024E] to hSET1/SET1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human hSET1/SET1 aa 1200-1250. The exact sequence is proprietary. Gene ID 9739.
Database link: O15047
General notes

This product is sold under License from Bethyl Laboratories, Inc.
This product was previously labelled as hSET1

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR024E
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSET1/SET1 antibody [BLR024E] (ab243881)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for hSET1/SET1 using ab243881 at 1/25 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSET1/SET1 antibody [BLR024E] (ab243881)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for hSET1/SET1 using ab243881 at 1/25 dilution in immunohistochemical analysis. A DyLight594-conjugated goat anti-rabbit antibody was used as the secondary. The nuclear counter stain is DAPI (blue)
Immunoprecipitation - Anti-hSET1/SET1 antibody [BLR024E] (ab243881)

hSET1/SET1 was immunoprecipitated from 1.0 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab243881 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243881 at 1/1000 dilution.

Lane 1: ab243881 IP in HEK-293T whole cell lysate.

Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Western blot - Anti-hSET1/SET1 antibody [BLR024E] (ab243881)

Western blot analysis using ab243881 at 1/1000 dilution.

Lane 1: HeLa whole cell lysate.

Lane 2: HEK-293T whole cell lysate .

Lane3: Jurkat whole cell lysate.

Lane 4: TCMK-1 whole cell lysate.

A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary.
Anti-hSET1/SET1 antibody [BLR024E] (ab243881)

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