Anti-HRP antibody (ab181658)
Key features and details
- Goat polyclonal to HRP
- Suitable for: WB
- Reacts with: Horseradish
- Isotype: IgG
Overview
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Product name
Anti-HRP antibody
See all HRP primary antibodies -
Description
Goat polyclonal to HRP -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Horseradish -
Immunogen
Full length native protein (purified) corresponding to HRP. (Horseradish).
Database link: P00433 -
Positive control
- Horseradish peroxidase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
ab181658 was purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HRP antibody (ab181658)All lanes : Anti-HRP antibody (ab181658) at 1/5000 dilution
Lane 1 : Horseradish peroxidase (reducing conditions) at 1 µg
Lane 2 : Horseradish peroxidase (reducing conditions) at 0.25 µg
Lane 3 : Horseradish peroxidase (non-reducing conditions) at 1 µg
Lane 4 : Horseradish peroxidase (non-reducing conditions) at 0.25 µg
Secondary
All lanes : Dylight 649 conjugated Donkey anti Goat at 1/10000 dilution
Predicted band size: 39 kDaReduced samples of purified Horseradish peroxidase contained 4% BME and were boiled for 5 minutes.
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Western blot - Anti-HRP antibody (ab181658)Anti-HRP antibody (ab181658) at 1/1000 dilution + Peroxidase at 0.05 µg
Secondary
DyLight™ 649 goat secondary at 1/20000 dilution
Predicted band size: 39 kDa
