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Anti-HRP antibody (ab181708)

Anti-HRP antibody (ab181708)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse polyclonal to HRP
  • Suitable for: WB
  • Reacts with: Horseradish
  • Isotype: IgG

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Overview

  • Product name

    Anti-HRP antibody
    See all HRP primary antibodies
  • Description

    Mouse polyclonal to HRP
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Horseradish
  • Immunogen

    Full length native protein (purified) corresponding to HRP aa 31-338.
    Sequence:

    QLTPTFYDNSCPNVSNIVRDTIVNELRSDPRIAASILRLHFHDCFVNGCD ASILLDNTTSFRTEKDAFGNANSARGFPVIDRMKAAVESACPRTVSCADL LTIAAQQSVTLAGGPSWRVPLGRRDSLQAFLDLANANLPAPFFTLPQLKD SFRNVGLNRSSDLVALSGGHTFGKNQCRFIMDRLYNFSNTGLPDPTLNTT YLQTLRGLCPLNGNLSALVDFDLRTPTIFDNKYYVNLEEQKGLIQSDQEL FSSPNATDTIPLVRSFANSTQTFFNAFVEAMDRMGNITPLTGTQGQIRLN CRVVNSNS


    Database link: P00433
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 0.88% Sodium chloride, 0.27% Potassium phosphate

    Does not contain preservative or stabilizer
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    ab181708 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the stated buffer stated. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum as well as purified and partially purified Peroxidase [Horseradish].
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • Conjugates

Images

  • Western blot - Anti-HRP antibody (ab181708)
    Western blot - Anti-HRP antibody (ab181708)
    Anti-HRP antibody (ab181708) at 1/1000 dilution (overnight at 4°C) + Peroxidase (Horseradish) at 0.1 µg

    Secondary
    Fluorescein conjugated mouse secondary antibody for 30 minutes at room temperature at 1/40000 dilution

    Predicted band size: 39 kDa
    Observed band size: 44 kDa
    why is the actual band size different from the predicted?



    Block: Blocking Buffer for Fluorescent Western Blotting for 30 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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